Animal gut microbiome mediates the effects of antibiotic pollution on an artificial freshwater system
Animal gut microbiome mediates the effects of antibiotic pollution on an artificial freshwater system
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动物肠道微生物组介导抗生素污染对人工淡水系统的影响
DOI：
10.1016/j.jhazmat.2021.127968
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发表时间：
2021
影响因子：
13.6
通讯作者：
Jiang Jianping
中科院分区：
文献类型：
--
作者：
Zhu Wei;Yang Duoli;Chang Liming;Zhang Meihua;Zhu Lifeng;Jiang Jianping
抗生素污染已成为一个全球性的环境问题，但其生态后果尚不清楚，尤其是抗生素与不同生态要素之间的相互作用还知之甚少。在这项研究中，三种代表性的抗生素，即，四环素，红霉素，磺胺甲恶唑，集中在一个简化的人工淡水系统组成的两栖类蝌蚪，肠道和环境的细菌和真菌群落，和水参数进行了研究。结果表明，抗生素暴露降低了蝌蚪的适合度，增加了死亡率和生理异常，并改变了水质，特别是氮稳态。在生物体、共生体和系统水平上的连续分析显示，抗生素破坏了蝌蚪的代谢组（例如，四氢生物蝶呤代谢）。抗生素还重塑了蝌蚪肠道细菌和真菌的多样性和组成，这部分解释了蝌蚪的健康状况。此外，蝌蚪肠道微生物组的变化（即，蓝藻和担子菌（BasidiomycotaOTUs）部分解释了水体参数的变化。与此相反，环境微生物和宏基因组保持相对稳定，并没有贡献的环境变化。这些结果强调了肠道微生物组在介导抗生素对宿主和环境的影响中的关键作用，这将扩大我们对抗生素污染造成的生态后果的理解。
The antibiotic pollution has become an emerging environmental problem worldwide, but the ecological outcomes remain to be elucidated, especially very little is known about the interactions between antibiotics and different ecological elements. In this study, the long-term influences of three representative antibiotics, i.e., tetracycline, erythromycin, and sulfamethoxazole, were investigated focusing on a simplified artificial freshwater system composed of amphibian tadpoles, gut and environmental bacterial and fungi communities, and water parameters. Results demonstrated that antibiotic exposure reduced tadpole’s fitness with increased mortality and physiological abnormality, and altered the water quality, particularly the nitrogen homeostasis. Sequential analyses at organism, symbiont, and systematic levels revealed that antibiotics disrupted tadpole metabolome (e.g., tetrahydrobiopterin metabolism) directly by off-target effects. Antibiotics also reshaped the tadpole gut bacterial and fungi diversity and composition, which partly accounted for the tadpole’s health condition. Moreover, changes of tadpole gut microbiome (i.e.,CyanobacteriaandBasidiomycotaOTUs) partly explained the variations of water parameters. In contrast, environmental microbiota and metagenome stayed relatively stable, and didn’t contribute to the environmental variations. These results highlighted the pivotal role of gut microbiome in mediating the effects of antibiotics on the host and the environment, which would extend our understanding on the ecological outcomes caused by antibiotic pollution.