Characterization of Φ8, a bacteriophage containing three double-stranded RNA genomic segments and distantly related to Φ6
Characterization of Φ8, a bacteriophage containing three double-stranded RNA genomic segments and distantly related to Φ6
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DOI：
10.1006/viro.2000.0374
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发表时间：
2000-06-20
期刊：
影响因子：
3.7
通讯作者：
Mindich, L
中科院分区：
文献类型：
--
作者：
Hoogstraten, D;Qiao, XY;Mindich, L
将噬菌体Phi-8的3个双链RNA基因组片段复制为cDNA，并测定了它们的核苷酸序列。尽管基因组的结构与Phi 6相似，但在核苷酸序列和氨基酸序列上都没有相似之处，除了在假定的聚合酶序列中发现的病毒RNA聚合酶的基序外。病毒蛋白的几个特征与phi 6显著不同。虽然这两个噬菌体都被一层含脂膜覆盖，但其蛋白质组成却非常不同。最显著的区别是，P8蛋白是pH8的膜的一部分，它构成了pH6中前衣壳的一个外壳。宿主附着蛋白由两个而不是一个肽组成，噬菌体直接结合到宿主的脂多糖上，而不是附着在IV型菌毛上。宿主范围包括鼠伤寒沙门氏菌粗株和假单胞菌(C)2000学术出版社。
The three double-stranded RNA genomic segments of bacteriophage Phi 8 were copied as cDNA, and their nucleotide sequences were determined. Although the organization of the genome is similar to that of Phi 6, there is no similarity in either the nucleotide sequences or the amino acid sequences, with the exception of the motifs characteristic of Viral RNA polymerases that are found in the presumptive polymerase sequence. Several features of the viral proteins differ markedly from those of Phi 6. Although both phages are covered by a lipid-containing membrane, the protein compositions are very different. The most striking difference is that protein P8, which constitutes a shell around the procapsid in Phi 6, is part of the membrane in Phi 8. The host attachment protein consists of two peptides rather than one and the phage attaches directly to the lipopolysaccharide of the host rather than to a type IV pilus. The host range of Phi 8 includes rough strains of Salmonella typhimurium and of pseudomonads (C) 2000 academic Press.