Comparative genomic analysis of three white spot syndrome virus isolates of different virulence
Comparative genomic analysis of three white spot syndrome virus isolates of different virulence
复制标题
三种不同毒力的白斑综合征病毒分离株的比较基因组分析
DOI：
10.1007/s11262-016-1421-z
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发表时间：
2017-04-01
期刊：
影响因子：
1.6
通讯作者：
Yang, Feng
中科院分区：
文献类型：
--
作者：
Li, Fang;Gao, Meiling;Yang, Feng
我们前期的研究中鉴定出三种不同毒力的白斑综合症病毒（WSSV）分离株，即高毒力株WSSV-CN01、中毒力株WSSV-CN02和低毒力株WSSV-CN03。在这项研究中，对这三种 WSSV 分离株的基因组进行了测序、注释和比较。 WSSV-CN01、WSSV-CN02 和 WSSV-CN03 的基因组大小分别为 309,286、294,261 和 284,148 bp，分别包含 177、164 和 154 个假定的蛋白质编码基因。研究了基因组变异，包括插入、缺失和取代。与 WSSV-CN01 相比，WSSV-CN02 或 WSSV-CN03 中的 34 个基因显示出 >20% 的序列变异，其中包括 6 个包膜蛋白基因（wsv237/vp41A、wsv238/vp52A、wsv338/vp62、wsv339/vp39、wsv077/vp36A 和wsv242/vp41B）和两个即早基因（wsv108 和 wsv178）。不同毒力的 WSSV 分离株之间的基因组变异，尤其是编码区的变异，无疑为理解 WSSV 发病机制的分子基础提供了新的见解。
Three white spot syndrome virus (WSSV) isolates of different virulence were identified in our previous study, the high-virulent strain WSSV-CN01, the moderate-virulent strain WSSV-CN02 and the low-virulent strain WSSV-CN03. In this study, the genomes of these three WSSV isolates were sequenced, annotated and compared. The genome sizes for WSSV-CN01, WSSV-CN02, and WSSV-CN03 are 309,286, 294,261, and 284,148 bp, bearing 177, 164, and 154 putative protein-coding genes, respectively. The genomic variations including insertions, deletions, and substitutions were investigated. Thirty four genes show >20% variation in their sequences in WSSV-CN02 or WSSV-CN03, in comparison with WSSV-CN01, including six envelope protein genes (wsv237/vp41A, wsv238/vp52A, wsv338/vp62, wsv339/vp39, wsv077/vp36A, and wsv242/vp41B), and two immediate-early genes (wsv108 and wsv178). The genomic variations among WSSV isolates of different virulence, especially those in the coding regions, certainly provide new insight into the understanding of the molecular basis of WSSV pathogenesis.