Double-strand breaks in DNA during somatic hypermutation of Ig genes: cause or consequence?
Double-strand breaks in DNA during somatic hypermutation of Ig genes: cause or consequence?
复制标题
Ig 基因体细胞超突变期间 DNA 双链断裂：原因还是结果？
DOI：
10.1016/s1471-4906(01)02112-3
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发表时间：
2002
影响因子：
16.8
通讯作者：
Pavlov,YouriI
中科院分区：
文献类型：
--
作者：
Rogozin,IgorB;Kunkel,ThomasA;Pavlov,YouriI
弗里德及其同事最近的一份报告中更详细地描述了艾滋病毒如何进出细胞。一段时间以来，人们就知道 HIV 蛋白 Gag 必须附着在细胞膜的内表面上，然后才能产生新病毒。现在，我们在这项研究中了解到，艾滋病毒必须附着在细胞膜上富含胆固醇的区域才能造成破坏。研究人员使用去除胆固醇或抑制其合成的化合物从富含胆固醇的斑块（筏）中耗尽胆固醇，发现这显着降低了艾滋病毒感染新细胞的能力。他们提出，任何帮助艾滋病毒导航并附着在筏上的机制都将有助于病毒传播，相反，即使筏的适度破坏也可能阻碍病毒进出宿主细胞的能力，从而减缓病毒传播。弗里德令人鼓舞地说道，“我们的发现清楚地证明了 Gag-raft 关联是 HIV 复制的关键步骤。需要进行额外的实验来确定是否可以通过治疗方法中断这种相互作用来治疗 HIV 感染者。’PNAS (2001) 98, 13925− 13930, NIH News Release AL
A more detailed picture of how HIV travels in and out of cells emerged in a recent report by Freed and colleagues. It has been known for some time that the HIV protein Gag must attach to the inner surface of the cell membrane before new virus can be produced. Now, we learn in this study that HIV has to attach to cholesterol-rich regions of the cell membrane before it can cause destruction. Researchers depleted cholesterol from cholesterol-rich patches (rafts), using compounds that remove cholesterol or inhibit its synthesis, and found that this reduced significantly the ability of HIV to infect new cells. They propose that any mechanism that helps HIV to navigate and attach to rafts would help the virus spread, and conversely, even a modest disruption of the rafts could hinder the ability of the virus to travel in and out of host cells, thereby slowing viral spread. Freed states encouragingly,‘Our findings are clear evidence that Gag–raft association is a critical step in HIV replication. Additional experiments are needed to determine whether this interaction can be interrupted therapeutically to treat HIV-infected people.’PNAS (2001) 98, 13925− 13930, NIH News Release AL