Lateral flow assays.
Lateral flow assays.
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DOI：
10.1042/ebc20150012
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发表时间：
2016-06-30
影响因子：
6.4
通讯作者：
Gallotta A
中科院分区：
文献类型：
--
作者：
Koczula KM;Gallotta A
横向流动测定法（LFAs）是生物医学、农业、食品和环境科学中流行的低成本、简单、快速和便携式检测设备背后的技术。本文综述了该方法的原理和检测的关键组成部分，重点介绍了侧流免疫检测。这种类型的分析最近引起了相当大的兴趣，因为它有可能直接向患者提供即时诊断。还将讨论用于评价测定的结果和参数的范围和解释。总结lfa的主要优点和缺点，并提出未来对检测设备和策略的相关改进。最后，将探讨LFA领域的主要最新进展和未来诊断应用。
Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays. This type of assay has recently attracted considerable interest because of its potential to provide instantaneous diagnosis directly to patients. The range and interpretation of results and parameters used for evaluation of the assay will also be discussed. The main advantages and disadvantages of LFAs will be summarized and relevant future improvements to testing devices and strategies will be proposed. Finally, the major recent advances and future diagnostic applications in the LFA field will be explored.