Appearance of vascular endothelial growth factor (VEGF) in femoral head in the growing rat.
Appearance of vascular endothelial growth factor (VEGF) in femoral head in the growing rat.
复制标题
生长大鼠股骨头中血管内皮生长因子（VEGF）的出现。
DOI：
10.14670/hh-16.463
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发表时间：
2001
影响因子：
2
通讯作者：
M. Yoshizuka
中科院分区：
文献类型：
--
作者：
M. Ichigatani;T. Saga;K. Yamaki;M. Yoshizuka
在这项研究中，我们研究了血管内皮生长因子（VEGF）的外观在生长的大鼠股骨头使用免疫细胞化学技术。结果表明，VEGF免疫阳性细胞存在于各个发育阶段的股骨头内区和外周区。在19日龄的胎儿，免疫阳性间充质细胞被证明在股骨头的周边区域。生后1 ~ 10天，在股骨的成骨细胞、破骨细胞、骨膜、软骨膜和软骨基质中观察到VEGF免疫反应阳性。生后15天，股骨头顶区出现VEGF免疫反应阳性的软骨细胞。在这个阶段，股骨头仍然由软骨细胞构成，没有观察到明显的血管形成。此后，股骨头中免疫阳性软骨细胞的数量增加。生后60天，股骨头韧带内可见毛细血管穿透。结果提示，在股骨头骨化开始前，部分软骨细胞产生VEGF，VEGF可能在股骨头韧带血管穿入股骨头中起重要作用。
In this study, we examined the appearance of vascular endothelial growth factor (VEGF) in the femoral head of the growing rat using an immunocytochemical technique. Our results showed VEGF-immunopositive cells existed in the inner region and peripheral region of the femoral head at each developmental stage. In the 19-day-old fetus, immunopositive mesenchymal cells were demonstrated in the peripheral region of the femoral head. At 1 to 10 days after birth, VEGF immunoreactivities were observed in the osteoblasts, osteoclasts, periosteum, perichondrium and cartilage matrix of the femur. At 15 days after birth, VEGF immunoreactive chondrocytes appeared in the apex area of the femoral head. In this stage, the femoral head is still constituted by chondrocytes and no apparent vascular formation has been observed. Thereafter, the immunopositive chondrocytes in the femoral head increased in number. The penetration of capillaries was recognized within the ligament of the femoral head at 60 days after birth. The results indicate that some chondrocytes in the femoral head produce VEGF before the beginning of ossification, and that VEGF may play an important role in the penetration of blood vessels into the femoral head from the ligament of the femoral head.
DOI：
10.1016/s0021-9258(18)42003-0
发表时间：
1992-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Claffey;W. Wilkison;B. Spiegelman
通讯作者：
K. Claffey;W. Wilkison;B. Spiegelman