Separation of inositol phosphates by high-performance ion-exchange chromatography.
Separation of inositol phosphates by high-performance ion-exchange chromatography.
复制标题
通过高效离子交换色谱法分离肌醇磷酸酯。
DOI：
10.1039/an9881300645
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
P. Mulholland
中科院分区：
文献类型：
--
作者：
R. Minear;J. Segars;J. Elwood;P. Mulholland
A method is presented for the liquid chromatographic separation of inositol phosphate (IP) esters on a strong anion-exchange column which eliminates the column hydrolysis that plagued earlier investigations. Acceptable separation conditions that resolve the various IP congeners consisted of a 60-min linear gradient from 0.1 to 0.5 M sodium chloride in 0.5 mM tetrasodium ethylenediaminetetraacetate. The method was applied to an enzymatic hydrolysate of inositol hexaphosphate (phytate), a wheat bran extract and a soil extract. The last sample was pre-treated by alkaline bromination prior to high-performance liquid chromatographic separation. Phosphorus-specific detection was accomplished by discrete fraction collection and manual analysis using persulphate oxidation and the ascorbic acid-molybdate phosphate method.
