Chemical modification of the inner and outer surfaces of Tobacco Mosaic Virus (TMV).

Chemical modification of the inner and outer surfaces of Tobacco Mosaic Virus (TMV).
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DOI:
10.1007/978-1-62703-751-8_13
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发表时间:
2014
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Steinmetz NF
Steinmetz NF
中科院分区:
其他
文献类型:
--
作者:
Bruckman MA;Steinmetz NF

文献摘要

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烟草花叶病毒(TMV)病毒纳米粒在各个领域有着广泛的应用。本文报道了TMV的纯化和化学工程。TMV是一种中空杆状的植物病毒纳米颗粒,具有可修饰的内、外表面。在这里,我们描述的方法,以隔离TMV从其烟草植物宿主的空间控制的内部和外部的化学修饰,并表征所得的TMV杂交材料。
Viral nanoparticles derive from tobacco mosaic virus (TMV) has broad applications in various fields. We report the purification and chemical engineering of TMV which is a hollow rod-shaped plant viral nanoparticle with modifiable interior and exterior surfaces. Here, we describe methods to isolate TMV from its tobacco plant host for spatially controlled interior and exterior chemical modification and to characterize the resulting TMV hybrid materials.