Hypoxia-induced metabolic and antioxidant enzymatic activities in the estuarine fish Leiostomus xanthurus
Hypoxia-induced metabolic and antioxidant enzymatic activities in the estuarine fish Leiostomus xanthurus
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DOI：
10.1016/s0022-0981(02)00329-5
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发表时间：
2002-11-06
影响因子：
2
通讯作者：
West, TL
中科院分区：
文献类型：
--
作者：
Cooper, RU;Clough, LM;West, TL
北卡罗来纳州的塔尔帕姆利科河流域经常是夏季鱼类死亡的地点。鱼类死亡的一个可能原因是缺氧引起的代谢应激。要检查缺氧诱导的压力在有机体和酶的水平，我们暴露于不同的氧张力（10%饱和度，0.8毫克/升; 25%，2.0毫克/升; 50%，4.0毫克/升; 100%，8.0毫克/升）的常见河口鱼Leiostomus xanthurus Lacepede（点）。暴露12 h后，提取鳃、肝脏和肌肉组织样品，随后分析代谢（柠檬酸合酶[CS]和乳酸脱氢酶[LDH]）和抗氧化剂（过氧化氢酶[CAT]和超氧化物歧化酶[SOD]）酶活性以及蛋白质含量。有一个显着的增加，乳酸脱氢酶活性，无氧代谢的指标，在10%的处理在鳃组织，而柠檬酸合酶活性，有氧代谢的指标没有显着的变化。抗氧化酶被证明是相互独立的，在10%饱和度下，鳃和肌肉组织中的超氧化物歧化酶活性显着增加，但在整个氧处理过氧化氢酶活性没有显着变化。在某些情况下，我们还注意到，氧处理内酶活性的个体间变异超过了处理间平均值的变异。缺氧似乎触发超氧化物歧化酶的活动在现场，但每个人的反应似乎是高度可变的，可能是由于先前暴露于缺氧。(C)2002 Elsevier Science B. V.保留所有权利。
The Tar-Pamlico River basin in North Carolina is regularly the site of summertime fish kills. One possible cause of fish kills is hypoxia-induced metabolic stress. To examine hypoxia-induced stress at both the organismal and enzymatic levels, we exposed the common estuarine fish Leiostomus xanthurus Lacepede (spot) to various oxygen tensions (10% saturation, 0.8 mg/l; 25%, 2.0 mg/l; 50%, 4.0 mg/l; 100%, 8.0 mg/l). After 12 h of exposure, samples of gill, liver, and muscle tissue were extracted and subsequently analyzed for metabolic (citrate synthase [CS] and lactate dehydrogenase [LDH]) and antioxidant (catalase [CAT] and superoxide dismutase [SOD]) enzyme activity as well as protein content. There was a significant increase in lactate dehydrogenase activity, an indicator of anaerobic metabolism, in the 10% treatment in gill tissue, while there was no significant change in citrate synthase activity, an indicator of aerobic metabolism. Antioxidant enzymes were shown to operate independently of one another, with significant increases in superoxide dismutase activity in gill and muscle tissue under 10% saturation but no significant change in catalase activity across oxygen treatments. In several cases, we also noted that interindividual variation of enzyme activities within oxygen treatments was in excess of the variation in means between treatments. Hypoxia appears to trigger superoxide dismustase activity in spot, but each individual's response seems to be highly variable, perhaps due to prior exposure to hypoxia. (C) 2002 Elsevier Science B.V. All rights reserved.