Groucho-associated transcriptional repressor ripply1 is required for proper transition from the presomitic mesoderm to somites
Groucho-associated transcriptional repressor ripply1 is required for proper transition from the presomitic mesoderm to somites
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DOI：
10.1016/j.devcel.2005.09.021
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发表时间：
2005-12-01
影响因子：
11.8
通讯作者：
Takada, S
中科院分区：
文献类型：
--
作者：
Kawamura, A;Koshida, S;Takada, S
伴随着从有体前中胚层向体节的转变，有体前中胚层的周期性基因表达特征发生了剧烈的变化，并转化为节段结构。然而，这种转变背后的分子机制仍然不清楚。在这里，我们表明，ripping 1，编码与转录辅阻遏物Groucho相关的核蛋白，是需要这种过渡。斑马鱼的riptarin 1在前PSM和几个新形成的体节中表达。Ripeptide 1通过Groucho相互作用基序抑制PSM中的mesp-b表达。在riptophorin 1缺陷的胚胎，体节边界不形成，PSM中的特征基因表达没有正确终止，并在节段单位最初建立的头尾极性不保持，而近轴中胚层细胞分化。因此，riptarin 1在从PSM到体节的过渡中起着双重作用：终止PSM中的分割程序和维持吻尾极性。
Concomitant with the transition from the presomitic mesoderm (PSM) to somites, the periodical gene expression characteristic of the PSM is drastically changed and translated into the segmental structure. However, the molecular mechanism underlying this transition has remained obscure. Here, we show that ripply1, encoding a nuclear protein associated with the transcriptional corepressor Groucho, is required for this transition. Zebrafish ripply1 is expressed in the anterior PSM and in several newly formed somites. Ripply1 represses mesp-b expression in the PSM through a Groucho-interacting motif. In ripply1-deficient embryos, somite boundaries do not form, the characteristic gene expression in the PSM is not properly terminated, and the initially established rostro-caudal polarity in the segmental unit is not maintained, whereas paraxial mesoderm cells become differentiated. Thus, ripply1 plays dual roles in the transition from the PSM to somites: termination of the segmentation program in the PSM and maintenance of the rostrocaudal polarity.