Expression and localization of polygalacturonase during the outgrowth of lateral roots in Allium porrum L.
Expression and localization of polygalacturonase during the outgrowth of lateral roots in Allium porrum L.
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DOI：
10.1007/bf00216810
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发表时间：
1992-09
期刊：
影响因子：
4.3
通讯作者：
R. Peretto;F. Favaron;V. Bettini;G. Lorenzo;S. Marini;P. Alghisi;F. Cervone;P. Bonfante
中科院分区：
文献类型：
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作者：
R. Peretto;F. Favaron;V. Bettini;G. Lorenzo;S. Marini;P. Alghisi;F. Cervone;P. Bonfante
研究了韭菜（Allium porrumL.）多聚半乳糖醛酸酶的存在及其与侧根形成的关系。苗进行了调查。在根的生长过程中，多聚半乳糖醛酸酶活性的稳步增加与侧根原基的活性。通过快速蛋白质液相色谱法对根提取物进行分级，分离出至少两种聚半乳糖醛酸酶亚型。其中一个亚型，75 kdalton蛋白，强烈反应的多克隆抗体对番茄多聚半乳糖醛酸酶的蛋白质印迹探测。它还与针对串珠镰孢多聚半乳糖醛酸酶产生的多克隆和单克隆抗血清反应。这三种抗体的原位定位结果表明，多聚半乳糖醛酸酶存在于侧根原基的分生组织上。抗果胶的抗体（Knox等，1990，Planta 181，512-521）检测到大量的果胶物质填充在原基顶端和母根组织之间的区域。我们认为多聚半乳糖醛酸酶在韭菜根的形态发生中起着重要的作用，特别是在侧根生长过程中。
The presence of polygalacturonase and its correlation with the formation of lateral roots in leek (Allium porrumL.) seedlings have been investigated. During root growth, a steady increase in polygalacturonase activity was associated with that of the lateral root primordia. Fractionation of root extract by fast protein liquid chromatography resolved at least two polygalacturonase isoforms. One of the isoforms, a 75-kdalton protein, strongly reacted on Western blots probed with a polyclonal antibody raised against tomato polygalacturonase. It also reacted with both polyclonal and monoclonal antisera raised againstFusarium moniliformepolygalacturonase. In situ localization with these three antibodies showed that polygalacturonase was present over the meristems of lateral root primordia. Antibodies against pectins (Knox et al. 1990, Planta181, 512–521) detected large amounts of pectic material filling the area between the apex of the primordium and the mother root tissues. We suggest that a polygalacturonase plays an important role in leek root morphogenesis, particularly during lateral root outgrowth.