An evolutionary and functional analysis of FoxL2 in rainbow trout gonad differentiation
An evolutionary and functional analysis of FoxL2 in rainbow trout gonad differentiation
复制标题
DOI：
10.1677/jme.1.01566
复制
发表时间：
2004-12-01
影响因子：
3.5
通讯作者：
Veitia, RA
中科院分区：
文献类型：
--
作者：
Baron, D;Cocquet, J;Veitia, RA
FOXL2是一种参与卵巢发育和功能的叉头转录因子。在这里，我们研究了FOXL2基因及其同源基因在鱼类中的进化和表达模式。我们发现了保守的FOXL2序列(FoxL2a)和差异基因，其叉头区属于L2类，并与FoxL2a序列(命名为FoxL2b)同源。在虹鳟鱼中，FoxL2a和FoxL2b在卵巢中特异表达，但表现出不同的时间表达模式。FoxL2a的表达与芳香酶的水平相关，芳香酶是产生雌激素的关键酶，雌激素治疗用于使遗传上的男性个体雌性化，引起了两个近亲动物的上调。相反，雄激素或芳香酶抑制剂下调女性FoxL2a和FoxL2b的表达。我们推测，雌激素和FOXL2在鱼类中的表达之间存在直接联系，至少在性腺对激素处理敏感的时期是这样。
FOXL2 is a forkhead transcription factor involved in ovarian development and function. Here, we have studied the evolution and pattern of expression of the FOXL2 gene and its paralogs in fish. We found well conserved FoxL2 sequences (FoxL2a) and divergent genes, whose forkhead domains belonged to the class L2 and were shown to be paralogs of the FoxL2a sequences (named FoxL2b). In the rainbow trout, FoxL2a and FoxL2b were specifically expressed in the ovary, but displayed different temporal patterns of expression. FoxL2a expression correlated with the level of aromatase, the key enzyme in estrogen production, and an estrogen treatment used to feminize genetically male individuals elicited the up-regulation of both paralogs. Conversely, androgens or an aromatase inhibitor down-regulated FoxL2a and FoxL2b in females. We speculate that there is a direct link between estrogens and FoxL2 expression in fish, at least during the period where the identity of the gonad is sensitive to hormonal treatments.