High Expression of Glucose Transporter 1 on Primary Lesions of Esophageal Squamous Cell Carcinoma is Associated with Hematogenous Recurrence
High Expression of Glucose Transporter 1 on Primary Lesions of Esophageal Squamous Cell Carcinoma is Associated with Hematogenous Recurrence
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DOI：
10.1245/s10434-013-3371-1
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发表时间：
2014-05-01
影响因子：
3.7
通讯作者：
Baba, Hideo
中科院分区：
文献类型：
--
作者：
Sawayama, Hiroshi;Ishimoto, Takatsugu;Baba, Hideo
目的。葡萄糖转运蛋白1型（Glut1）在多种恶性肿瘤的肿瘤特异性代谢中起着至关重要的作用，以适应肿瘤的快速生长和肿瘤微环境。本研究探讨了Glut1在食管鳞状细胞癌原发病变中的临床、病理和预后特征。对145例术前未治疗的可切除食管鳞状细胞癌患者的组织进行石蜡包埋切片，对Glut1和CD34进行免疫组化染色。CD34染色计算微血管密度。41例患者（28.2%）出现Glut1阳性，并与侵袭深度相关[比值比（OR） 2.984；95%置信区间（CI） 1.208 ~ 7.371；P = 0.018]和血管侵犯（OR 2.771; 95% CI 1.118 ~ 6.871; P = 0.028）。Glut1阳性对无复发生存均有显著不利[危险比（HR） 2.021；95% ci 1.100-3.712；P = 0.023]和食管癌特异性生存（HR 2.223; 95% CI 1.121-4.411; P = 0.022），但在多因素分析中与无复发生存或癌症特异性生存没有独立相关。研究Glut1表达与首次复发部位的关系。在单因素Cox风险分析中，Glut1阳性与淋巴结复发无相关性（HR 1.009; 95% CI 0.402-2.530; P = 0.985），但与血液复发有显著相关性（HR 3.701; 95% CI 1.655-8.273; P = 0.001）。计算微血管密度评价血管生成情况，发现Glut1阳性与微血管密度高相关（P < 0.001）。Glut1表达与血液性复发相关。这些发现为Glut1表达作为生物标志物的重要性提供了证据。
Purpose. Glucose transporter type 1 (Glut1) plays a crucial role in cancer-specific metabolism to adapt to the rapid growth and tumor microenvironment in diverse malignant tumors. This study examined the clinical, pathological, and prognostic features of Glut1 expression on primary lesions of esophageal squamous cell carcinoma.Methods. Immunohistochemical staining of Glut1 and CD34 was performed using paraffin-embedded sections of tissues obtained from 145 resectable esophageal squamous cell carcinoma patients without preoperative treatment. Microvessel density was calculated from CD34 staining.Results. Glut1 positivity was observed in 41 patients (28.2 %) and associated with depth of invasion [odds ratio (OR) 2.984; 95 % confidence interval (CI) 1.208-7.371; P = 0.018] and vascular invasion (OR 2.771; 95 % CI 1.118-6.871; P = 0.028) in multivariate analysis. Glut1 positivity was a significant disadvantage to both relapse-free survival [hazard ratio (HR) 2.021; 95 % CI 1.100-3.712; P = 0.023] and esophageal cancer-specific survival (HR 2.223; 95 % CI 1.121-4.411; P = 0.022) in univariate Cox hazard analysis, but was not independently associated with relapse-free survival or cancer-specific survival in multivariate analysis. The relationship between Glut1 expression and first relapse site was investigated. Glut1 positivity was not associated with lymph node recurrence (HR 1.009; 95 % CI 0.402-2.530; P = 0.985) but was significantly associated with hematogenous recurrence (HR 3.701; 95 % CI 1.655-8.273; P = 0.001) in univariate Cox hazard analysis. Microvessel density was calculated to evaluate angiogenesis, and it was observed that Glut1 positivity was significantly associated with high microvessel density (P < 0.001).Conclusions. Glut1 expression was associated with hematogenous recurrence. The findings provide evidence of the significance of Glut1 expression as a biomarker.