Nek2A contributes to tumorigenic growth and possibly functions as potential therapeutic target for human breast cancer
Nek2A contributes to tumorigenic growth and possibly functions as potential therapeutic target for human breast cancer
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DOI：
10.1002/jcb.24059
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发表时间：
2012-06-01
影响因子：
4
通讯作者：
Niu, Yun
中科院分区：
文献类型：
--
作者：
Wang, Shuling;Li, Weidong;Niu, Yun
Nek 2A（NIMA相关激酶2A）是一种重要的中心体调节因子。本研究旨在探讨Nek 2A在乳腺癌中的表达及其在不同阶段乳腺癌中的作用。我们检测了MCF-10细胞系包括MCF-10A、MCF-10DCIS.com、MCF-10 CA 1a和人乳腺样品中Nek 2A在mRNA和蛋白水平上的表达，所述人乳腺样品包含正常乳腺组织（NBT）、乳腺导管原位癌（DCIS）和浸润性导管癌（IDC）。我们的研究显示，Nek 2A的mRNA和蛋白表达在MCF-10DCIS.com和MCF-10 CA 1a细胞系以及人原发性乳腺癌组织（DCIS和IDC）中显著上调。本研究还揭示了Nek 2A mRNA表达与临床病理因素的相关性。Nek 2A mRNA表达与ER、PR、Ki-67免疫反应性及分子亚型有关（P < 0. 05）。
Nek2A (NIMA-related kinases 2A) has been known as an important centrosome regulatory factor. The aim of this study was to investigate the expression of Nek2A and the role it played in different stages of breast cancer. We detected the expression of Nek2A in both mRNA and protein levels in MCF10 cell lines including MCF-10A, MCF-10DCIS.com, MCF-10CA1a and in human breast samples which contained normal breast tissue (NBT), breast ductal carcinoma in situ (DCIS), and invasive ductal carcinoma (IDC). Our study revealed that the mRNA and protein expression of Nek2A were significantly up-regulated in MCF-10DCIS.com and MCF-10CA1a cell lines as well as in human primary breast cancer tissue (DCIS and IDC). Our study also presented a correlation between Nek2A mRNA expression and some clinic pathological factors. We found that Nek2A mRNA expression was associated with molecular subtypes, ER, PR and Ki-67 immunoreactivity (P?