Use of zonal centrifugation for preparing synchronous cultures from Ehrlich ascites cells grown in vivo.
Use of zonal centrifugation for preparing synchronous cultures from Ehrlich ascites cells grown in vivo.
复制标题
使用区域离心从体内生长的艾利希腹水细胞制备同步培养物。
DOI：
10.1016/0014-4827(73)90077-3
复制
发表时间：
1973
影响因子：
3.7
通讯作者：
J. Maisenbacher
中科院分区：
文献类型：
--
作者：
H. Probst;J. Maisenbacher
描述了一种允许选择属于细胞周期不同阶段的艾氏腹水肿瘤细胞的方法。细胞在体内生长，从小鼠腹腔内取出后立即通过区域离心分离。来自通过区带离心获得的不同级分的细胞的进一步体外生长表明，该方法允许制备同步培养物，所述同步培养物在细胞周期的不同点开始生长并且在至少两个另外的循环中保持同步。选择过程似乎会导致随后出现细胞周期缩短，这种细胞周期在异步原代培养物中逐渐发展。该方法的优点是容量大且起始材料易于获得。
A method is described which permits the selection of Ehrlich ascites tumour cells belonging to different stages of the cell cycle. The cells are grown in vivo and separated by zonal centrifugation immediately after being withdrawn from the intraperitoneal cavity of mice. Further in vitro growth of cells derived from different fractions obtained by zonal centrifugation demonstrates that this method permits the preparation of synchronous cultures which begin to grow at different points of the cell cycle and which maintain synchrony for at least two further cycles. The selection procedure seems to cause the subsequent appearance of a shortened cell cycle which develops more gradually in asynchronous primary cultures. Advantages of this method are, the large capacity and easy availability of the starting material.