Fragile X-related protein and VIG associate with the RNA interference machinery
Fragile X-related protein and VIG associate with the RNA interference machinery
复制标题
DOI：
10.1101/gad.1025202
复制
发表时间：
2002-10-01
影响因子：
10.5
通讯作者：
Hammond, SM
中科院分区：
文献类型：
--
作者：
Caudy, AA;Myers, M;Hammond, SM
RNA干扰（RNAi）是一种灵活的基因沉默机制，通过抑制同源基因来响应双链RNA。在这里，我们报告了包含小干扰 RNA 和 microRNA (miRNA) 的 RNAi 效应复合物 (RISC) 的表征。我们通过与 RISC 的关联，鉴定了两种假定的 RNA 结合蛋白，即脆性 X 智力迟钝蛋白 (FMRP) 的果蝇同源物、dFXR 和 VIG（Vasa 内含子基因）。 FMRP 是人类脆弱 X 基因座的产物，通过未知机制调节大量 mRNA 的表达。 dFXR 和潜在的 FMRP 至少部分使用 RNAi 相关机制进行目标识别的可能性表明 RNAi 与人类疾病之间存在潜在的重要联系。
RNA interference (RNAi) is a flexible gene silencing mechanism that responds to double-stranded RNA by suppressing homologous genes. Here, we report the characterization of RNAi effector complexes (RISCs) that contain small interfering RNAs and microRNAs (miRNAs). We identify two putative RNA-binding proteins, the Drosophila homolog of the fragile X mental retardation protein (FMRP), dFXR, and VIG (Vasa intronic gene), through their association with RISC. FMRP, the product of the human fragile X locus, regulates the expression of numerous mRNAs via an unknown mechanism. The possibility that dFXR, and potentially FMRP, use, at least in part, an RNAi-related mechanism for target recognition suggests a potentially important link between RNAi and human disease.