An effective and recyclable deproteinization method for polysaccharide from oyster by magnetic chitosan microspheres
An effective and recyclable deproteinization method for polysaccharide from oyster by magnetic chitosan microspheres
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磁性壳聚糖微球有效且可回收的牡蛎多糖脱蛋白方法
DOI：
10.1016/j.carbpol.2018.04.108
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发表时间：
2018-09-01
影响因子：
11.2
通讯作者：
Li, Shijie
中科院分区：
文献类型：
--
作者：
Song, Zhuoyue;Hu, Youdong;Li, Shijie
制备磁性壳聚糖微球（MCM）用于近江牡蛎粗多糖（ORP）的脱蛋白，并对其吸附性能进行评价。首先，采用微乳液法合成了MCM。采用傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、能谱仪（EDS）、X射线衍射仪（XRD）和振动样品磁强计（VSM）对样品进行了表征。最后，将MCM作为磁性可分离吸附剂应用于氧化还原酶的脱蛋白。结果表明，MCM表面光滑，粒径为2-6 μ m。吸附动力学和吸附等温线分别符合准二级动力学方程和Freundlich方程。与Sevag法相比，MCM法脱蛋白率高，多糖回收率高，污染少。此外，脱蛋白能力可以再生。因此，MCM是一种很有前途的多糖脱蛋白吸附剂。
The aim of this study was to synthesize magnetic chitosan microspheres (MCM) for the deproteinization of crude polysaccharides from Ostrea rivularis Gould (ORP), and evaluate their adsorption properties. Firstly, MCM were synthesized by microemulsion process. Then they were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and vibrating-sample magnetometer (VSM). At last, MCM was applied as a magnetic separable adsorbent for deproteinization of ORP. The results showed that MCM had smooth surface with particle diameter of 2-6 mu m. The adsorption kinetics and adsorption isotherms were well fitted by the pseudo-second order equation and the Freundlich equation, respectively. Comparing with the Sevag method, MCM exhibited higher deproteinization ratio, higher polysaccharides recovery, and miner pollution. In addition, the deprotenaization capacity can be regenerated. Therefore, MCM would be used as promising adsorbents for the deproteinization of polysaccharides.