New colored and fluorescent amine substrates for activated fibrin stabilizing factor (Factor XIIIa) and for transglutaminase.
New colored and fluorescent amine substrates for activated fibrin stabilizing factor (Factor XIIIa) and for transglutaminase.
复制标题
用于激活纤维蛋白稳定因子（因子 XIIIa）和转谷氨酰胺酶的新型有色荧光胺底物。
DOI：
10.1016/0003-2697(83)90193-8
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发表时间：
1983
影响因子：
2.9
通讯作者：
SiefringJr,GE
中科院分区：
文献类型：
--
作者：
Lorand,L;Parameswaran,KN;Velasco,PT;Hsu,LK;SiefringJr,GE
为了扩大标记蛋白质中某些γ-谷氨酰胺位点的可能性，在本实验室早期用丹磺酰尸胺进行工作之后，合成了两种荧光（FITC和6-氯-2-甲氧基吖啶）和一种强烈着色的尸胺衍生物（dabsyl）。测定了胺底物对人凝血因子XIII a和豚鼠肝转氨酶的酶亲和力。通过琼脂糖电泳后对这些酶进行活性染色，并通过比色法测量人血浆中的因子XIII酶原，进一步证明了dabsylcadarine的效用。
Two fluorescent (FITC and 6-chloro-2-methoxyacridine) and an intensely colored (dabsyl) derivative of cadaverine were synthesized, following earlier work from this laboratory with dansylcadaverine, in order to enlarge the scope of possibilities for labeling some γ-glutamine sites in proteins. Enzyme affinities of the amine substrates for human Factor XIIIaand for guinea pig liver transglutaminase were measured. The utility of dabsylcadaverine was further demonstrated by activity staining of these enzymes, following electrophoresis in agarose, and by measuring the Factor XIII zymogen of human plasma colorimetrically.