Bacterial endosymbiont of the slender pigeon louse, Columbicola columbae, allied to endosymbionts of grain weevils and tsetse flies
Bacterial endosymbiont of the slender pigeon louse, Columbicola columbae, allied to endosymbionts of grain weevils and tsetse flies
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DOI：
10.1128/aem.01131-07
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发表时间：
2007-10-01
影响因子：
4.4
通讯作者：
Clayton, Dale H.
中科院分区：
文献类型：
--
作者：
Fukatsu, Takema;Koga, Ryuichi;Clayton, Dale H.
目前的研究集中在一种共生细菌中发现的细长鸽虱，哥伦比亚（昆虫纲：Phthiraptera）。分子系统发育分析表明，该共生体属于变形菌纲的γ亚门，与舌蝇（Glossina spp.）以及谷物象鼻虫（Sitophilus spp.）的初级共生体。相对速率检验表明，C.与采采蝇共生体和象鼻虫共生体相比，柱虫表现出加速的分子进化。整体安装在原位杂交被用来本地化的共生体，并确定在主机发展的感染动力学。在第一和第二龄幼虫中，共生体定位于卵圆形细菌细胞的细胞质中，在体腔两侧形成小的聚集体。在三龄幼虫中，细菌细胞迁移到中央体，并最终位于侧输卵管的前部区域，形成称为卵巢壶腹的明显组织结构。在成年雌性中，共生体从卵巢壶腹传递到卵巢管中发育的卵母细胞。在成年男性中，细菌细胞经常消失而不迁移。对从日本、美国、澳大利亚和阿根廷收集的昆虫进行的诊断PCR调查检测到96.5%（109/113）的感染，少数未感染的雄性昆虫。这项研究提供了第一个微生物特征的细菌细胞相关的共生体从咀嚼虱。共生体可能的生物学作用进行了讨论，与宿主的营养生理与羽毛喂养的生活方式。
The current study focuses on a symbiotic bacterium found in the slender pigeon louse, Columbicola columbae (Insecta: Phthiraptera). Molecular phylogenetic analyses indicated that the symbiont belongs to the gamma subdivision of the class Proteobacteria and is allied to Sodalis glossinidius, the secondary symbiont of tsetse flies (Glossina spp.) and also to the primary symbiont of grain weevils (Sitophilus spp.). Relative-rate tests revealed that the symbiont of C. columbae exhibits accelerated molecular evolution in comparison with the tsetse fly symbiont and the weevil symbiont. Whole-mount in situ hybridization was used to localize the symbiont and determine infection dynamics during host development. In first- and second-instar nymphs, the symbionts were localized in the cytoplasm of oval bacteriocytes that formed small aggregates on both sides of the body cavity. In third-instar nymphs, the bacteriocytes migrated to the central body and were finally located in the anterior region of the lateral oviducts, forming conspicuous tissue formations called ovarial ampullae. In adult females, the symbionts were transmitted from the ovarial ampullae to developing oocytes in the ovarioles. In adult males, the bacteriocytes often disappeared without migration. A diagnostic PCR survey of insects collected from Japan, the United States, Australia, and Argentina detected 96.5% (109/113) infection, with a few uninfected male insects. This study provides the first microbial characterization of a bacteriocyte-associated symbiont from a chewing louse. Possible biological roles of the symbiont are discussed in relation to the host nutritional physiology associated with the feather-feeding lifestyle.