LUTEINIZING-HORMONE HAS A ROLE IN DEVELOPMENT OF FULLY FUNCTIONAL CORPORA-LUTEA (CL) BUT IS NOT REQUIRED TO MAINTAIN CL FUNCTION IN HEIFERS
LUTEINIZING-HORMONE HAS A ROLE IN DEVELOPMENT OF FULLY FUNCTIONAL CORPORA-LUTEA (CL) BUT IS NOT REQUIRED TO MAINTAIN CL FUNCTION IN HEIFERS
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DOI：
10.1095/biolreprod51.6.1248
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发表时间：
1994-12-01
影响因子：
3.6
通讯作者：
KINDER, JE
中科院分区：
文献类型：
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作者：
PETERS, KE;BERGFELD, EG;KINDER, JE
我们测试的假设，内源性脉冲LH有一个作用，在发展和维护CL在发情周期的牛女性。通过间隔11天用前列腺素F-2 α处理两次，使20头小母牛同步发情（第0天=行为发情）。然后将小母牛随机分配至四种处理中的一种（n = 5/组）。用LHRH拮抗剂治疗小母牛（LHRH-Ant; N-Ac-D-Nal[2]（1），4Cl-D-Phe（2），D-Cit [3]（3），D-Cit（6），D-Ala（10）-LHRH; 10 μ g/kg体重）或载体（5%甘露醇）每24小时一次：1）LHRH-Ant第2-7天，2）LHRH-Ant第7-12天，3）LHRH-Ant第12-17天，4）无LHRH-Ant（对照）。在第0-24天每天两次从颈静脉采集血样，并从每个给药期开始至CL死亡或第24天（以先发生者为准）表征发情周期黄体期循环中孕酮曲线下的面积。当三个连续样本中孕酮含量低于1 ng/ml血浆时，认为发生了黄体溶解。在发情周期的黄体期的循环中的孕酮的轮廓下的面积进行了比较，从对照组的小母牛在同一时期。与对照相比，LHRH-Ant处理减少了所有处理组中的LH脉冲（p < 0.05）。在第2-7天用LHRH-Ant治疗降低了CL的功能（分别为3.72 +/- 0.93对7.36 +/- 1.02单位; p < 0.05）。在第7-12天用LHRH-Ant处理的小母牛也具有降低的CL功能（分别为3.02 +/- 0.33对6.75 +/- 0.99单位; p < 0.01）。然而，在第12-17天用LHRH-Ant治疗不影响CL的功能（分别为3.97 +/- 1.02对4.27 +/- 0.80单位; p > 0.10）。这些数据支持我们的假设，内源性脉冲LH有一个作用，在发展和维护CL在发情周期的牛女性。
We tested the hypothesis that endogenous pulses of LH have a role in development and maintenance of CL during the estrous cycle of the bovine female. Twenty heifers were synchronized to estrus by treating two times with prostaglandin F-2 alpha 11 days apart (Day 0 = behavioral estrus). Heifers were then randomly assigned to one of four treatments (n = 5/group). Heifers were treated with an antagonist to LHRH (LHRH-Ant; N-Ac-D-Nal[2](1),4Cl-D-Phe(2),D-Pal[3](3),D-Cit(6),D-Ala(10)-LHRH; 10 mu g/kg body weight) or vehicle (5% mannitol) once every 24 h: 1)LHRH-Ant Days 2-7, 2) LHRH-Ant Days 7-12, 3) LHRH-Ant Days 12-17, 4) no LHRH-Ant (control). Blood samples were collected from the jugular vein twice daily on Days 0-24, and area under the profile of progesterone in circulation during the luteal phase of the estrous cycle was characterized from the start of each treatment period until the demise of CL or Day 24, whichever came first. Luteolysis was considered to have occurred when three consecutive samples contained less than 1 ng progesterone/ml plasma. Areas under the profile of progesterone in circulation during the luteal phase of the estrous cycle were compared to those of heifers from the control group for the same period. LHRH-Ant treatment diminished LH pulses in all treatment groups compared to control (p < 0.05). Treatment with LHRH-Ant on Days 2-7 diminished function of CL (3.72 +/- 0.93 vs. 7.36 +/- 1.02 units, respectively; p < 0.05). Heifers treated with LHRH-Ant on Days 7-12 also had reduced function of CL (3.02 +/- 0.33 vs. 6.75 +/- 0.99 units, respectively; p < 0.01). However, treatment with LHRH-Ant on Days 12-17 did not influence function of CL (3.97 +/- 1.02 vs. 4.27 +/- 0.80 units, respectively; p > 0.10). The data support our hypothesis that endogenous pulses of LH have a role in development and maintenance of CL during the estrous cycle of bovine females.