LOCAL LEVELS OF INTERLEUKIN-1β, -4, -6, AND TUMOR NECROSIS FACTOR α IN AN EXPERIMENTAL MODEL OF MURINE OSTEOMYELITIS DUE TO STAPHYLOCOCCUS AUREUS
LOCAL LEVELS OF INTERLEUKIN-1β, -4, -6, AND TUMOR NECROSIS FACTOR α IN AN EXPERIMENTAL MODEL OF MURINE OSTEOMYELITIS DUE TO STAPHYLOCOCCUS AUREUS
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DOI：
10.1006/cyto.2002.1039
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发表时间：
2002-07
期刊：
影响因子：
3.8
通讯作者：
T. Yoshii;Shinya Magara;D. Miyai;Hidetaka Nishimura;Eiji Kuroki;S. Furudoi;T. Komori;C. Ohbayashi
中科院分区：
文献类型：
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作者：
T. Yoshii;Shinya Magara;D. Miyai;Hidetaka Nishimura;Eiji Kuroki;S. Furudoi;T. Komori;C. Ohbayashi
摘要本研究的目的是评估小鼠骨髓炎模型中局部白细胞介素1β（IL-1β）、4（IL-4）、6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平。用酶联免疫吸附法测定骨匀浆上清液中细胞因子的水平。金黄色。感染骨组织中IL-1β和IL-6的水平在感染后早期升高，然后下降。相比之下，TNF-α水平在感染后3至28天保持升高，而IL-4水平在感染过程后期升高。感染后3 ~ 7 d，骨组织病理学检查可见炎性细胞浸润和骨吸收，14 ~ 28 d可见骨吸收和邻近骨形成。这些结果表明，感染诱导的IL-1β和IL-6水平升高可能主要与感染早期的骨损伤有关，TNF-α和IL-4可能至少部分与该骨髓炎模型后期的组织病理学变化有关，包括骨吸收和骨形成。
Abstract The purpose of this study is to evaluate local levels of interleukin-1β (IL-1β), -4 (IL-4), -6 (IL-6), and tumour necrosis factor-α (TNF-α), in a model of murine osteomyelitis due to Staphylococcus aureus . Cytokine levels in supernatants derived from bone homogenates were determined by enzyme-linked immunosorbent assay, for 28 days following the direct implantation of murine tibiae with S. aureus . Levels of IL-1βand IL-6 in infected bone were elevated in the early post-infection period and then decreased. In contrast, TNF-αlevels remained elevated 3 to 28 days post-infection, while IL-4 levels were elevated late in the course of infection. The histopathology of infected bone showed predominant infiltration of inflammatory cells and bone resorption 3 to 7 days after infection, and bone resorption and adjacent areas of formation 14 to 28 days after infection. These results suggest that the elevated IL-1βand IL-6 levels induced by infection may be related to bone damage mainly in the early phase of infection, and that TNF-αand IL-4 may at least in part be associated with histopathological changes, including both bone resorption and formation in the later phase of this osteomyelitis model.