Characterization of the chicken PKR: polymorphism of the gene and antiviral activity against vesicular stomatitis virus.
Characterization of the chicken PKR: polymorphism of the gene and antiviral activity against vesicular stomatitis virus.
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发表时间：
2004-02
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通讯作者：
J. Ko;A. Asano;Y. Kon;Tomomasa Watanabe;T. Agui
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作者：
J. Ko;A. Asano;Y. Kon;Tomomasa Watanabe;T. Agui
干扰素诱导小鼠和人细胞产生双链RNA依赖的蛋白激酶(PKR)。在本研究中，我们克隆并测定了不同品种鸡PKR基因的核苷酸序列。鸡PKR是一个由开放阅读框架(ORF)推导出的550个氨基酸的蛋白质，在RNA结合蛋白和蛋白激酶家族中存在特定的结构域(两个双链RNA结合域(DRBD)和大量的激酶亚域)。进一步分析表明，鸡PKR基因具有一定的多态性。除Koshamo 3型(KS-3)外，表达鸡PKR基因的细胞克隆对水泡性口炎病毒均有抗病毒作用。KS-3PKR在507位氨基酸(精氨酸到谷氨酸氨基)发生了取代，表现出两栖抗病毒反应。这种特定的氨基酸替代被认为是确定鸡PKR的抗病毒功能的除了DRBD和KK亚区等必需结构域之外。
The double-stranded RNA-dependent protein kinase (PKR) is induced in mouse and human cells on treatment with interferon. In this study, we have cloned and determined the nucleotide sequences of chicken PKR cDNA in various chicken breeds. Chicken PKR was a 550-amino-acid protein as deduced from the cDNA open reading frame (ORF), and there were specific domains (two double-stranded RNA binding domains (DRBDs) and numerous kinase subdomains) characterized in RNA binding proteins and kinase families. Furthermore, it was suggested that chicken PKR was polymorphic. Transfected cell clones expressing chicken PKR mRNA were demonstrated to confer antiviral responses to vesicular stomatitis virus, except for Koshamo type-3 (KS-3). KS-3 PKR, which has an amino acid substitution at position 507 (Arg to Gln), showed amphibious antiviral responses. This specific amino acid substitution was considered to determine the antiviral function of chicken PKR in addition to essential domains as DRBDs and kinase subdomains.