Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis
Local database and the search program for proteomic analysis of sperm proteins in the ascidian Ciona intestinalis
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DOI：
10.1016/j.bbrc.2004.05.118
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发表时间：
2004-07-09
影响因子：
3.1
通讯作者：
Inaba, K
中科院分区：
文献类型：
--
作者：
Hozumi, A;Satouh, Y;Inaba, K
通过双向电泳和随后的质谱（MS）分离蛋白质现在是蛋白质组学分析的常规技术。为了对蛋白质初级结构信息有限的特定组织进行蛋白质组学分析，我们开发了一种对海鞘睾丸基因产物进行肽质量指纹图谱分析的系统。用双向凝胶电泳法分离琼脂精蛋白，用MALDI-TOF/MS法分离色氨酸片段。在在线数据库中对质量模式进行了搜索，但对这些蛋白质的鉴定较少。我们已经建立了一个质谱数据库，包括Ciona testis ESTs和基因组草图序列，以及一个新设计的基于perl的肽质量指纹图谱搜索程序PerMS。该系统可识别80%以上的乔娜精子蛋白，可广泛应用于基因组信息较少的有限组织的蛋白质组学分析。(C) 2004爱思唯尔公司版权所有。
Separation of proteins by two-dimensional electrophoresis and following mass spectrometry (MS) is now a conventional technique for proteomic analysis. For proteomic analysis of a certain tissue with a limited information of primary structures of proteins, we have developed an analytical system for peptide mass fingerprinting in gene products in the testis of the ascidian Ciona intestinalis. Ciona sperm proteins were separated by two-dimensional gel electrophoresis and the tryptic fragments were subjected to MALDI-TOF/MS. The mass pattern was searched against on-line databases but resulted in less identification of these proteins. We have constructed a MS database from Ciona testis ESTs and the genome draft sequence, along with a newly devised, perl-based search program PerMS for peptide mass fingerprinting. This system could identify more than 80% of Ciona sperm proteins, suggesting that it could be widely applied for proteomic analysis for a limited tissue with less genomic information. (C) 2004 Elsevier Inc. All rights reserved.