Characterization of single crystals of the large ribosomal particles from a mutant of Bacillus stearothermophilus.
Characterization of single crystals of the large ribosomal particles from a mutant of Bacillus stearothermophilus.
复制标题
嗜热脂肪芽孢杆菌突变体大核糖体颗粒单晶的表征。
DOI：
10.1016/0022-2836(86)90473-0
复制
发表时间：
1986
影响因子：
5.6
通讯作者：
Wittmann,HG
中科院分区：
文献类型：
--
作者：
Yonath,A;Saper,MA;Frolow,F;Makowski,I;Wittmann,HG
单一的，三维晶体的50 S核糖体亚基，从突变体的嗜热脂肪芽孢杆菌缺乏蛋白质L11，其特征在于使用同步辐射X射线源。突变颗粒的晶体在相同的条件下生长，并且与相同细菌的野生型的晶体同晶。它们是斜方晶系，包含至少一个2重螺旋轴，并且具有a= 350（±10）A Ω，B= 670（±10）A Ω和c= 910（±10）A Ω的晶胞尺寸。它们在4° C时的分辨率为15至18 μ m，在同步加速器光束中稳定数小时。
Single, three-dimensional crystals of 50S ribosomal subunits, from a mutant of Bacillus stearothermophilus that lacks the protein L11, have been characterized using a synchrotron X-ray source. The crystals of the mutated particles grow under the same conditions and are isomorphous to those of the wild type of the same bacteria. They are orthorhombic, contain at least one 2-fold screw axis, and have unit cell dimensions of a= 350 (±10) A ̊, b= 670 (±10) A ̊, and c= 910 (±10) A ̊. They diffract to 15 to 18 Å resolution at 4° C and are stable in the synchrotron beam for several hours.