Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast
Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast
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DOI：
10.1074/jbc.m508985200
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发表时间：
2006-01-27
影响因子：
4.8
通讯作者：
Dohlman, HG
中科院分区：
文献类型：
--
作者：
Wang, YQ;Dohlman, HG
生物学中的一个基本问题是不同的信号通路如何使用共同的信号蛋白来获得不同的发育结果。酵母转录因子Ste 12在至少两个不同的信号传导过程中是必需的，每个过程由许多相同的蛋白激酶调节。Ste 12-Ste 12同源二聚体促进交配所需基因的转录，而Ste 12-Tec 1异源二聚体激活侵入性生长所需的基因。我们报告说，Ste 12和Tec 1进行共价修饰的泛素相关的修饰SUMO。刺激交配信息素促进类小泛素化的Ste 12和减少类小泛素化的Tec 1。在不存在类小泛素化的情况下，Tec 1降解得更快。我们建议，信息素调节的SUMO化的STE 12和Tec 1促进了发展开关从入侵到交配分化程序。
A fundamental question in biology is how different signaling pathways use common signaling proteins to attain different developmental outcomes. The yeast transcription factor Ste12 is required in at least two distinct signaling processes, each regulated by many of the same protein kinases. Whereas Ste12-Ste12 homodimers promote transcription of genes required for mating, Ste12-Tec1 heterodimers activate genes required for invasive growth. We report that Ste12 and Tec1 undergo covalent modification by the ubiquitin-related modifier SUMO. Stimulation by mating pheromone promotes sumoylation of Ste12 and diminishes the sumoylation of Tec1. In the absence of sumoylation Tec1 is more rapidly degraded. We propose that pheromone-regulated sumoylation of Ste12 and Tec1 promotes a developmental switch from the invasive to the mating differentiation program.