Biosensing Of A Herbicide Using A Chemically Modified Antibody In Organic Solvent
Biosensing Of A Herbicide Using A Chemically Modified Antibody In Organic Solvent
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在有机溶剂中使用化学修饰抗体对除草剂进行生物传感
DOI：
10.1080/00032719708001792
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发表时间：
1997
影响因子：
2
通讯作者：
I. Karube
中科院分区：
文献类型：
--
作者：
S. Sasaki;Y. Tokitsu;K. Ikebukuro;K. Yokoyama;Y. Masuda;I. Karube
摘要报道了有机溶剂中阿特拉津与2，4-二（邻甲氧基聚乙二醇）-6-氯均三嗪修饰的抗阿特拉津抗体的反应。用活化的PEG 2（2，4-双（邻甲氧基聚乙二醇）-6-氯-s-三嗪）修饰被证明对抗体的结合亲和力没有影响。用活化的PEG 2修饰的抗体在甲苯中的溶解度为26 μg/mL。PEG修饰的抗阿特拉津抗体固定在可控孔玻璃（CPG）上，在有机溶剂中对阿特拉津具有亲和性，并提出了应用于酶免疫分析的可能性。
ABSTRACT The reaction in organic solvent between atrazine and anti-atrazine antibody modified with 2,4-bis(o-methoxy-polyethylene glycol)-6-chloro-s-triazine was reported. Modification with activated PEG2 (2,4-bis(o-methoxypolyethylene glycol)-6-chloro-s-triazine) was demonstrated to have no influence on the binding affinity of the antibody. Solubility of the antibody modified with activated PEG2 in toluene was 26 μg/mL. PEG modified anti-atrazine antibody immobilized onto Controlled Pore Glass (CPG) was suggested to have affinity towards atrazine in the organic solvent and the possibility of application for the enzyme immunoassay was suggested.