Loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum.
Loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum.
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DOI：
10.1111/j.1469-8137.2012.04129.x
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发表时间：
2012-07
期刊：
影响因子：
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通讯作者：
L. Voll;Martina B. Zell;Timo Engelsdorf;Alexandra Saur;M. G. Wheeler;M. Drincovich;A. Weber;V. Maurino
中科院分区：
文献类型：
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作者：
L. Voll;Martina B. Zell;Timo Engelsdorf;Alexandra Saur;M. G. Wheeler;M. Drincovich;A. Weber;V. Maurino
•虽然光合nadp -苹果酸酶（NADP-ME）在C(4)循环中发挥着重要作用，但非光合同工型的生物学功能仍然难以捉摸。在这里，我们分析了拟南芥细胞质NADP-ME2与植物防御反应之间的联系。•利用病原体相关分子模式（pathogen-associated molecular patterns, PAMPs）诱导和与半生物营养真菌病原菌Colletotrichum higginsianum相互作用，分析了野生型和修饰型拟南芥NADP-ME2表达水平。•在正常生长条件下，NADP-ME2活性的缺乏或增加会导致植物代谢物池大小的巨大变化，而对形态或发育没有任何影响。经PAMP处理和病原菌感染后，NADP-ME总活性和NADP-ME2转录物水平均增强。在感染C. higginsianum期间，NADP-ME2的功能缺失突变体（NADP-ME2）表现出增强的易感性。nadp-me2抑制诱导后瞬时体外活性氧（ROS）的产生和感染后胼胝质乳头的形成。晚期水杨酸依赖性和非水杨酸依赖性防御反应不受影响。综上所述，我们的研究结果表明，NADP-ME2在植物基础防御中起着重要作用，它似乎参与了ROS的产生。此外，NADP-ME2被发现对后期的防御反应是必不可少的。
• While photosynthetic NADP-malic enzyme (NADP-ME) has a prominent role in the C(4) cycle, the biological function of nonphotosynthetic isoforms remains elusive. Here, we analysed the link between Arabidopsis thaliana cytosolic NADP-ME2 and the plant defence response. • Arabidopsis thaliana plants with wild-type and modified NADP-ME2 expression levels were analysed after elicitation with pathogen-associated molecular patterns (PAMPs) and during the interaction with the hemibiotrophic fungal pathogen Colletotrichum higginsianum. • Under normal growth conditions, the lack or gain of NADP-ME2 activity produced large changes in plant metabolite pool sizes without any effect on morphology or development. Total NADP-ME activity and NADP-ME2 transcript level were enhanced after PAMP treatment and pathogen infection. During infection with C. higginsianum, loss-of-function mutants of NADP-ME2 (nadp-me2) showed enhanced susceptibility. Transient apoplastic reactive oxygen species (ROS) production after elicitation and callose papilla formation after infection were dampened in nadp-me2. Late salicylic acid (SA)-dependent and SA-independent defence responses were not affected. • Taken together, our results indicate that NADP-ME2 is an important player in plant basal defence, where it appears to be involved in the generation of ROS. Moreover, NADP-ME2 was found to be dispensable for later defence responses.