Comprehensive Morphological Analysis of Individual Peripheral Neuron Dendritic Arbors in Ascidian Larvae using the Photoconvertible Protein Kaede
Comprehensive Morphological Analysis of Individual Peripheral Neuron Dendritic Arbors in Ascidian Larvae using the Photoconvertible Protein Kaede
复制标题
使用光转换蛋白 Kaede 对海鞘幼虫个体周围神经元树突状乔木进行综合形态学分析
DOI：
10.1002/dvdy.24169
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发表时间：
2014
影响因子：
2.5
通讯作者：
Kotaro Oka
中科院分区：
文献类型：
--
作者：
Takatoshi D Yokoyama;Kohji Hotta;Kotaro Oka
背景：海鞘幼虫表皮感觉神经元（ESNs）的每一个延伸到外部的身体结构，或被膜，形成一个独特的外部感觉网络，ASNET（海鞘树突网络在被膜）。ESNs和ASNET的功能尚不清楚，尽管有人认为它们在游泳幼虫中起着重要作用。在这项研究中，我们使用转基因幼虫表达的光转换蛋白Kaede pan‐ neuronalto确定ASNET.Results内的单个ESN外部过程的方向和形态：当单个ESN细胞体的UV‐照射，光转换的Kaede蛋白扩散到外突，突出到边缘的被膜，表明这些过程是细胞质连接到ESN的细胞体。因此，我们能够全面地分类每个ESN外部过程的形态和方向。大多数干ESNs似乎延长神经突起的触须，但没有过程中观察到从触须神经元发出。ESN过程在其形态和方向上系统地不同，这表明ASNET是以一种受调节的、非随机的方式形成的。结论：这项研究揭示了海鞘幼虫被膜中ESN感觉场的组织。发育动力学243：1362-1373，2014。© 2014 Wiley Periodicals，Inc.
Background:Ascidian larval epidermal sensory neurons (ESNs) each extend a single cilium into the outer body structure, or tunic, to form a unique external sensory network, the ASNET (ascidian dendritic network in tunic). The functions of ESNs and the ASNET are unknown, though it has been suggested that they play important roles in swimming larvae. In this study, we used transgenic larva expressing the photoconvertible protein Kaede pan‐neuronally to identify the orientation and morphology of single ESN external processes within the ASNET.Results:When individual ESN cell bodies were UV‐irradiated, the photoconverted Kaede protein diffused into the external processes that projected to the edge of the tunic, indicating that these processes are cytoplasmically connected to the cell bodies of ESNs. We were, therefore, able to comprehensively catalog the morphology and orientation of each ESN external process. Most trunk ESNs appeared to extend neurites to the palp, but no processes were observed to emanate from palp neurons. ESN processes differed systematically in their morphology and orientation, suggesting that the ASNET is formed in a regulated, non‐random fashion.Conclusions:This study reveals the organization of ESN sensory fields in the ascidian larval tunic.Developmental Dynamics 243:1362–1373, 2014. © 2014 Wiley Periodicals, Inc.