N-acetylcysteine stimulates protein synthesis in enterocytes independently of glutathione synthesis
N-acetylcysteine stimulates protein synthesis in enterocytes independently of glutathione synthesis
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N-乙酰半胱氨酸刺激肠细胞中的蛋白质合成，独立于谷胱甘肽合成
DOI：
10.1007/s00726-015-2105-z
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发表时间：
2015-10
期刊：
影响因子：
3.5
通讯作者：
Wu Guoyao
中科院分区：
文献类型：
--
作者：
Yi Dan;Hou Yongqing;Wang Lei;Long Minhui;Hu Shengdi;Mei Huimin;Yan Liqiong;Hu Chien-An Andy;Wu Guoyao
Dietary supplementation with N-acetylcysteine (NAC) has been reported to improve intestinal health and treat gastrointestinal diseases. However, the underlying mechanisms are not fully understood. According to previous reports, NAC was thought to exert its effect through glutathione synthesis. This study tested the hypothesis that NAC enhances enterocyte growth and protein synthesis independently of cellular glutathione synthesis. Intestinal porcine epithelial cells were cultured for 3 days in Dulbecco’s modified Eagle medium containing 0 or 100 μM NAC. To determine a possible role for GSH (the reduced form of glutathione) in mediating the effect of NAC on cell growth and protein synthesis, additional experiments were conducted using culture medium containing 100 μM GSH, 100 μM GSH ethyl ester (GSHee), diethylmaleate (a GSH-depletion agent; 10 μM), or a GSH-synthesis inhibitor (buthionine sulfoximine, BSO; 20 μM). NAC increased cell proliferation, GSH concentration, and protein synthesis, while inhibiting proteolysis. GSHee enhanced cell proliferation and GSH concentration without affecting protein synthesis but inhibited proteolysis. Conversely, BSO or diethylmaleate reduced cell proliferation and GSH concentration without affecting protein synthesis, while promoting protein degradation. At the signaling level, NAC augmented the protein abundance of total mTOR, phosphorylated mTOR, and phosphorylated 70S6 kinase as well as mRNA levels for mTOR and p70S6 kinase in IPEC-1 cells. Collectively, these results indicate that NAC upregulates expression of mTOR signaling proteins to stimulate protein synthesis in enterocytes independently of GSH generation. Our findings provide a hitherto unrecognized biochemical mechanism for beneficial effects of NAC in intestinal cells.
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影响因子：
7.4
作者：
E. Ruiz;R. Siow;S. Bartlett;A. Jenner;Hideyo Sato;S. Bannai;G. Mann
通讯作者：
E. Ruiz;R. Siow;S. Bartlett;A. Jenner;Hideyo Sato;S. Bannai;G. Mann
影响因子：
--
作者：
Lai, Zhi-Wei;Hanczko, Robert;Bonilla, Eduardo;Caza, Tiffany N.;Clair, Brandon;Bartos, Adam;Miklossy, Gabriella;Jimah, John;Doherty, Edward;Tily, Hajra;Francis, Lisa;Garcia, Ricardo;Dawood, Maha;Yu, Jianghong;Ramos, Irene;Coman, Ioana;Faraone, Stephen V.;Phillips, Paul E.;Perl, Andras
通讯作者：
Perl, Andras
影响因子：
2.8
作者：
Romagnoli C;Marcucci T;Picariello L;Tonelli F;Vincenzini MT;Iantomasi T
通讯作者：
Iantomasi T
影响因子：
4.2
作者：
Wu, GY;Fang, YZ;Turner, ND
通讯作者：
Turner, ND
影响因子：
4
作者：
Lan Yang;Panpan Tan;W. Zhou;Xu Zhu;Yong-yao Cui;Liang Zhu;Xue-mei Feng;H. Qi;Jun Zheng
通讯作者：
Lan Yang;Panpan Tan;W. Zhou;Xu Zhu;Yong-yao Cui;Liang Zhu;Xue-mei Feng;H. Qi;Jun Zheng