Nitric oxide improves membrane fluidity of erythrocytes in essential hypertension: An electron paramagnetic resonance investigation.
Nitric oxide improves membrane fluidity of erythrocytes in essential hypertension: An electron paramagnetic resonance investigation.
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DOI：
10.1006/bbrc.2000.3408
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发表时间：
2000-09
影响因子：
3.1
通讯作者：
K. Tsuda;K. Kimura;I. Nishio;Y. Masuyama
中科院分区：
文献类型：
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作者：
K. Tsuda;K. Kimura;I. Nishio;Y. Masuyama
已有研究表明，血液流变性异常可能是高血压的病因之一。最近的研究表明，人红细胞具有一氧化氮合酶，这种激活可能参与了红细胞流变性的调节。本研究用电子顺磁共振(EPR)和自旋标记方法研究了NO在高血压病患者红细胞膜功能调节中的作用。NO供体S-亚硝基-N-乙酰青霉胺(SNAP)使红细胞膜EPR谱测定的5-硝基硬脂酸(5-NS)的序参数(S)和16-NS的峰高比(h(0)/h(-1))呈剂量依赖性降低。结果表明，NO供体使红细胞膜流动性增加。此外，8-溴-环鸟苷一磷酸可显著增强SNAP的作用。相反，在L-N(G)-硝基精氨酸甲酯和不对称二甲基L-精氨酸存在下，SNAP引起的流动性变化被逆转。高血压病患者红细胞膜流动性明显低于正常血压组。SNAP对高血压病患者的影响比正常血压受试者更明显。结果表明，NO增加了细胞膜的流动性，降低了细胞膜的刚性。此外，NO对高血压病患者血液流动性的影响更大，提示NO可能积极参与红细胞流变性的调节，在改善高血压患者微循环中起重要作用。
It has been shown that rheological abnormality might be an etiological factor in hypertension. Recent studies have revealed that human erythrocytes possess a nitric oxide (NO) synthase and that this activation might be involved in the regulation of rheological properties of erythrocytes. The present study was undertaken to investigate the role of NO in the regulation of membrane functions of erythrocytes in patients with essential hypertension by means of an electron paramagnetic resonance (EPR) and spin-labeling method. The NO donor S-nitroso-N-acetylpenicillamine (SNAP) decreased the order parameter (S) for 5-nitroxide stearate (5-NS) and the peak height ratio (h(0)/h(-1)) for 16-NS obtained from EPR spectra of erythrocyte membranes in a dose-dependent manner. The finding indicated that the NO donor increased the membrane fluidity of erythrocytes. In addition, the effect of SNAP was significantly potentiated by 8-bromo-cyclic guanosine monophosphate. By contrast, the change of the fluidity induced by SNAP was reversed in the presence of L-N(G)-nitroarginine methyl ester and asymmetric dimethyl L-arginine. In patients with essential hypertension, the membrane fluidity of erythrocytes was significantly lower than in the normotensive subjects. The effect of SNAP was more pronounced in essential hypertension than in normotensive subjects. These results showed that NO increased the membrane fluidity and decreased the rigidity of cell membranes. Furthermore, the greater effect of NO on the fluidity in essential hypertension suggests that NO might actively participate in the regulation of rheological behavior of erythrocytes and have a crucial role in the improvement of microcirculation in hypertension.