Intra-accumbal Tat1-72 alters acute and sensitized responses to cocaine
Intra-accumbal Tat1-72 alters acute and sensitized responses to cocaine
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DOI：
10.1016/j.pbb.2008.05.020
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发表时间：
2008-10-01
影响因子：
3.6
通讯作者：
Booze, R. M.
中科院分区：
文献类型：
--
作者：
Harrod, S. B.;Mactutus, C. F.;Booze, R. M.
达特，HIV-1蛋白，静脉注射可卡因诱导的自发活动的影响进行了检查，在卵巢切除大鼠。使动物习惯于活动室，给予IV基线/盐水注射，24小时后，接受双侧注射。脑内微量注射达特（1-72）（15 μ g/μ l）或赋形剂。24小时后，大鼠接受14次每日IV可卡因注射（3.0 mg/kg/inj，1次/天）或盐水中的第一次。在基线后以及第1次和第14次可卡因注射后，在自动室中测量自发活动30分钟。还进行了可卡因后的观察时间采样。急性可卡因/生理盐水后，达特显着增加可卡因诱导的总活动超过30分钟的会议，在中央室的活动没有显着影响。重复注射可卡因产生的行为敏化与2倍高水平的总活动，类似于3倍高水平的中枢定向活动，并通过直接观察增加运动评分。重复使用可卡因/盐水后，达特改变了可卡因诱导的总活动行为致敏的发展，而之前的达特暴露则减弱了可卡因诱导的致敏的发展。总的来说，这些数据表明，双边微量注射达特到N Acc改变静脉可卡因诱导的行为，表明达特产生的行为变化，通过破坏中皮质边缘通路。(C)2008年爱思唯尔公司All rights reserved.
The effects of Tat, an HIV-1 protein, on intravenous cocaine-induced locomotor activity were examined in ovariectomized rats. Animals were habituated to activity chambers, administered an IV baseline/saline injection, and 24 h later, received bilateral. intra-accumbal microinjections of Tat(1-72) (15 mu g/mu l) or vehicle. Twenty four hours later, rats received the first of 14 daily IV cocaine injections (3.0 mg/kg/inj, 1/day) or saline. Locomotor activity was measured in automated chambers for 30 min following baseline and after the 1st and 14th cocaine injections. Observational time sampling following cocaine was also performed. Following acute cocaine/saline, Tat significantly increased cocaine-induced total activity over the 30-min session, with no significant effects for activity in the central compartment. Repeated cocaine injections produced behavioral sensitization with similar to 2-fold higher levels of total activity, similar to 3-fold higher levels of centrally directed activity, and increased locomotor scores via direct observations. Following repeated cocaine/saline, Tat altered the development of cocaine-induced behavioral sensitization for total activity with prior Tat exposure attenuating the development of cocaine-induced sensitization. Collectively, these data show that bilateral microinjection of Tat into the N Acc alters IV cocaine-induced behavior, suggesting that Tat produces behavioral changes by disrupting the mesocorticolimbic pathway. (C) 2008 Elsevier Inc. All rights reserved.