Assessment of chlorinated pesticides and polychlorinated biphenyls in adipose breast tissue using a supercritical fluid extraction method.
Assessment of chlorinated pesticides and polychlorinated biphenyls in adipose breast tissue using a supercritical fluid extraction method.
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使用超临界流体萃取方法评估脂肪乳腺组织中的氯化农药和多氯联苯。
DOI：
10.1093/carcin/15.11.2581
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发表时间：
1994
期刊：
影响因子：
4.7
通讯作者：
Stellman,SD
中科院分区：
文献类型：
--
作者：
Djordjevic,MV;Hoffmann,D;Fan,J;Prokopczyk,B;Citron,ML;Stellman,SD
开发了一种精确且重现性高的分析方法，用于评估脂肪组织中有机氯农药和多氯联苯残留（≥ 50 mg）。该方法可用于这些环境污染物在乳腺癌病因学中的意义的化学研究。采用超临界CO2和改性CO2（添加5%二氯甲烷）萃取（SFE）从乳腺癌患者和对照者手术切除的脂肪组织中去除所产生的农药残留。氧化铝吸附剂，放置在提取容器中与标本一起，除去大部分的共提取的脂质;随后纯化的SFE提取物的柱层析氧化铝去除剩余的微量脂质，这将干扰气相色谱分析与电子捕获检测。该方法是通过分析一个认证的参考材料430猪脂肪与已知量的农药残留物，通常发现在脂肪或高脂肪含量的食品进行测试。分析物的回收率从异狄氏剂的73.4%到α-、β-和γ-六氯环己烷、六氯苯和狄氏剂的115%不等，单个分析物的标准偏差为4-12%。根据这种新方法对脂肪组织中的有机氯化合物进行的分析表明，乳腺癌患者体内的农药含量高于对照组。然而，本研究中分析的样本数量较少（n= 5，两组），无法得出明确的结论。病例组和对照组中含量最高的化合物均为p，p-DDE（379 ± 286和160 ± 149 p.p.b.）和PCB（223 ± 145和124 ± 65.7 p.p.b.），其次是杀白蚁剂三氯杀螨酯残留物氧氯草胺和三甲基异丙甲草胺。
A precise and highly reproducible analytical method was developed for the assessment of organochlorinated pesticide and polychlorinated biphenyl residues in adipose tissue (≥ 50 mg). The method can be utilized for epidemlologlcal studies on the significance of these environmental pollutants in the etiology of breast cancer. Supercritical fluid extrac tion (SFE) with CO2and modified CO2(addition of 5% dichloromethane) Is employed to remove incurred pesticide residues from adipose tissues that have been surgically removed from breast cancer patients and controls. An alumina sorbent, placed in the extracting vessel together with a specimen, removes the bulk of co-extracted lipids; a subsequent purification of the SFE extracts by column chromatography on alumina removes the remaining traces of lipids that would interfere with the gas chromatographlc analysis with electron capture detection. The method was tested by analyzing a Certified Reference MaterIal 430 pork fat with known amounts of pesticide residues that are commonly found in fat or in foods with a high fat content. The recoveries of analytes ranged from 73.4% for endrin to 115% for α-, β- and γ-hexachlorocydohexane, hexachlorobenzene and dietdrin, with standard deviations of 4–12% for individual analytes. The analysis of adipose tissue for organochlorinated compounds on the basis of this new method suggested that the pesticide levels were higher in breast cancer patients than in controls. However, the small number of samples analyzed In this study (n= 5, both groups) precludes definitive conclusions. The most abundant compounds in both cases and controls werep,p-DDE (379 ± 286 and 160 ± 149 p.p.b.) and PCB (223 ± 145 and 124 ± 65.7 p.p.b.), followed by the termiticlde chlordane residues oxychlordane and tmnsnonachlor.