Development of the endoplasmic reticulum, mitochondrion and apicoplast during the asexual life cycle of Plasmodium falciparum
Development of the endoplasmic reticulum, mitochondrion and apicoplast during the asexual life cycle of Plasmodium falciparum
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DOI：
10.1111/j.1365-2958.2005.04699.x
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发表时间：
2005-07-01
影响因子：
3.6
通讯作者：
McFadden, GI
中科院分区：
文献类型：
--
作者：
van Dooren, GG;Marti, M;McFadden, GI
疟原虫是单细胞真核生物，在跨越两种宿主（蚊子和人）的多阶段生命周期过程中经历一系列显著的形态学转变。相对较少的是知道整个过程中的细胞器的动态这些转变。在这里，我们描述了三个细胞器（内质网，apicoplast和speacon）的形态，通过人体血液阶段的寄生虫生命周期使用荧光报告蛋白融合到细胞器靶向序列。内质网最初是一个简单的新月形细胞器，发展成一个有两个小突起的核周环，随着寄生虫的扩大，内质网转变成一个广泛的网状网络。类似地，顶质体和寄生体从单个的、小的、离散的细胞器生长到后期寄生虫的高度分支的结构。这些分支结构经历了有序的分裂-顶质体随后是顶质体-以产生多个子细胞器，这些子细胞器显然是成对连接的，以包装成子细胞。这是第一次深入研究这种重要的人类病原体的无性生命周期中活寄生虫的细胞内细胞器。
Plasmodium parasites are unicellular eukaryotes that undergo a series of remarkable morphological transformations during the course of a multistage life cycle spanning two hosts (mosquito and human). Relatively little is known about the dynamics of cellular organelles throughout the course of these transformations. Here we describe the morphology of three organelles (endoplasmic reticulum, apicoplast and mitochondrion) through the human blood stages of the parasite life cycle using fluorescent reporter proteins fused to organelle targeting sequences. The endoplasmic reticulum begins as a simple crescent-shaped organelle that develops into a perinuclear ring with two small protrusions, followed by transformation into an extensive reticulated network as the parasite enlarges. Similarly, the apicoplast and the mitochondrion grow from single, small, discrete organelles into highly branched structures in later-stage parasites. These branched structures undergo an ordered fission - apicoplast followed by mitochondrion - to create multiple daughter organelles that are apparently linked as pairs for packaging into daughter cells. This is the first in-depth examination of intracellular organelles in live parasites during the asexual life cycle of this important human pathogen.