DNA replication timing influences gene expression level.
DNA replication timing influences gene expression level.
复制标题
DOI：
10.1083/jcb.201701061
复制
发表时间：
2017-07-03
期刊：
影响因子：
--
通讯作者：
Nieduszynski CA
中科院分区：
文献类型：
--
作者：
Müller CA;Nieduszynski CA
真核生物基因组按照可重现的时间顺序进行复制，其生理意义尚不清楚。米勒（Müller）和尼杜辛斯基（Nieduszynski）比较了系统发育上不同的酵母物种中基因组复制的时间顺序，并确定了保守的复制时间有助于其最大表达的基因。
真核生物基因组按照可重现的时间顺序进行复制；然而，其生理意义却鲜为人知。我们比较了不同酵母物种的复制时间，并确定了具有保守复制时间的基因组特征。在所有物种中，组蛋白基因都属于最早复制的位点。我们特意延迟了HTA1 - HTB1的复制，发现这使得这些组蛋白基因的表达减半。最后，我们表明，一般来说，组蛋白和细胞周期基因不受Rtt109依赖的剂量补偿的影响，这表明存在将特定基因座排除在剂量补偿机制之外的途径。因此，我们揭示了调控复制时间的首批生理需求之一，并证明了复制时间和基因表达之间的直接联系。
Eukaryotic genomes are replicated in a reproducible temporal order whose physiological significance is poorly understood. Müller and Nieduszynski compare the temporal order of genome replication in phylogenetically diverse yeast species and identify genes for which conserved replication timing contributes to maximal expression. Eukaryotic genomes are replicated in a reproducible temporal order; however, the physiological significance is poorly understood. We compared replication timing in divergent yeast species and identified genomic features with conserved replication times. Histone genes were among the earliest replicating loci in all species. We specifically delayed the replication of HTA1-HTB1 and discovered that this halved the expression of these histone genes. Finally, we showed that histone and cell cycle genes in general are exempt from Rtt109-dependent dosage compensation, suggesting the existence of pathways excluding specific loci from dosage compensation mechanisms. Thus, we have uncovered one of the first physiological requirements for regulated replication time and demonstrated a direct link between replication timing and gene expression.