Interaction of alpha-acetoxy-N-nitrosopyrrolidine with DNA assessed by loss of restriction endonuclease recognition sites and formation of apurinic/apyrimidinic sites.
Interaction of alpha-acetoxy-N-nitrosopyrrolidine with DNA assessed by loss of restriction endonuclease recognition sites and formation of apurinic/apyrimidinic sites.
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通过限制性内切核酸酶识别位点的丧失和无嘌呤/无嘧啶位点的形成来评估 α-乙酰氧基-N-亚硝基吡咯烷与 DNA 的相互作用。
DOI：
10.1093/carcin/9.9.1595
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发表时间：
1988
期刊：
影响因子：
4.7
通讯作者：
Archer,MC
中科院分区：
文献类型：
--
作者：
Gunhouse,BW;Milligan,JR;Archer,MC
α-乙酰氧基-N-亚硝基吡咯烷（AcO-NPYR）在酯酶存在下与质粒pBR 322反应。AcO-NPYR处理的质粒通过与亚精胺反应而产生切口，表明存在脱嘌呤位点。处理过的质粒也阻断了限制性内切核酸酶Bg/I的作用，并在较小程度上阻断了DraI的作用。这些限制性内切酶的识别序列表明AcO-NPYR优先与DNA中的鸟嘌呤和/或胞嘧啶碱基相互作用。
α-Acetoxy-N-nitrosopyrrolidine (AcO-NPYR), in the presence of esterase, was reacted with the plasmid pBR322. The AcO-NPYR-treated plasmid was nicked by reaction with spermidine, suggesting the presence of apurinic sites. The treated plasmid also blocked the action of the restriction endonucleaseBg/I and, to a lesser extent,DraI. The recognition sequences of these restriction enzymes suggest that AcO-NPYR interacts preferentially with guanine and/or cytosine bases in DNA.