Temporal position of G(1) arrest in normal human fibroblasts after exposure to gamma-rays
Temporal position of G(1) arrest in normal human fibroblasts after exposure to gamma-rays
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DOI：
10.1006/excr.1997.3496
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发表时间：
1997-04-10
影响因子：
3.7
通讯作者：
Lehnert, BE
中科院分区：
文献类型：
--
作者：
Gadbois, DM;Lehnert, BE
在具有野生型p53的细胞中已经记录了暴露于电离辐射后的G（1）期细胞周期停滞。然而，在G（1）阶段的时间位置尚未确定。我们现在已经使用溴脱氧尿苷（BrdUrd）标记的细胞的流式细胞仪分析，以获得关于G（1）期辐射检查点位置的进一步信息。用γ射线照射人成纤维细胞并用秋水仙胺处理，以阻止未标记的G（2）细胞进入G（1）期。对BrdUrd掺入的分析显示，在暴露于γ射线后，73%的G（1）期人肺成纤维细胞保持在G（1）期，从而将G（1）辐射检查点置于G（1）期结束附近。放射检查点的位置与报道的细胞周期蛋白E表达增加、细胞周期蛋白E/cdk 2激酶活性增加和增殖的人成纤维细胞中pRb过度磷酸化相关。(C)北京：科学出版社.
G(1) phase cell cycle arrest after exposure to ionizing radiation has been documented in cells with wild-type p53. The temporal location of this arrest within G(1) phase, however, has not been determined. We have now used flow cytometric analysis of bromodeoxyuridine (BrdUrd)-labeled cells to obtain further information about the location of the G(1) phase radiation checkpoint. Human fibroblasts were irradiated with gamma-rays and treated with colcemid to stop unlabeled G(2) cells from entering the G(1) phase. Analysis of BrdUrd incorporation revealed that 73% of G(1) phase human lung fibroblasts remain in G(1) phase after exposure to gamma-rays, thereby placing the G(1) radiation checkpoint near the end of G(1) phase. The location of the radiation checkpoint correlates with the reported increased expression of cyclin E, increased cyclin E/cdk2 kinase activity, and hyperphosphorylation of pRb in proliferating human fibroblasts. (C) 1997 Academic Press.