THE STRUCTURE AND ORGANIZATION OF THE BILE CANALICULAR CYTOSKELETON WITH SPECIAL REFERENCE TO ACTIN AND ACTIN-BINDING PROTEINS
THE STRUCTURE AND ORGANIZATION OF THE BILE CANALICULAR CYTOSKELETON WITH SPECIAL REFERENCE TO ACTIN AND ACTIN-BINDING PROTEINS
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DOI：
10.1002/hep.1840210433
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发表时间：
1995-04-01
期刊：
影响因子：
13.5
通讯作者：
PHILLIPS, MJ
中科院分区：
文献类型：
--
作者：
TSUKADA, N;ACKERLEY, CA;PHILLIPS, MJ
测定正常人肝细胞胆小管（BC）中肌动蛋白丝和肌动蛋白结合蛋白的分布，作为建立BC细胞骨架结构和组织的一种手段，免疫印迹显示存在肌动蛋白和肌动蛋白结合蛋白，肌动蛋白II，原肌动蛋白，血管蛋白，α -肌动蛋白，绒毛蛋白，以及非肌动蛋白相关蛋白-微管蛋白和细胞角蛋白。微绒毛核心丝，膜相关的微丝网络，以及环周的管周肌动蛋白丝带，肌动蛋白结合蛋白在这些区域与肌动蛋白非随机相关。在管周带的情况下，还与小带粘附连接，插入桥粒的中间丝，肌动蛋白结合蛋白的超微结构定位从根本上与微管相关微丝结构的排列和组织有关，细胞骨架的结构组织也与细胞间连接的不同组成部分有关。值得注意的是，原肌球蛋白和a-肌动蛋白是肌肉细胞中收缩活性的调节蛋白，肌动蛋白II与环管肌动蛋白微丝带有关；它与其他分泌细胞的顶端区域发现的收缩肌动蛋白丝带相对应，非收缩中间丝的外鞘可能稳定小管室。
The distribution of actin filaments and actin-binding proteins in the bile canaliculus (BC) of normal human hepatocytes was determined as a means of establishing the structure and organization of the BC cytoskeleton, Immunoblots demonstrated that actin, and the actin-binding proteins, myosin II, tropomyosin, vinculin, alpha-actinin, villin, were present, as were the non-actin-related proteins beta-tubulin, and cytokeratins, Three actin filament regions were identified: microvillus core filaments, a membrane-associated microfilamentous network, and a circumferential pericanalicular actin filament band, Actin-binding proteins were nonrandomly associated with actin in these regions, In the case of the pericanalicular band, there was also association with the zonula adherens junction, Intermediate filaments inserted into desmosomes, The ultrastructural localization of the actin-binding proteins was fundamentally Linked to the arrangement and organization of the major canaliculus-associated microfilament structures, Structural organization of the cytoskeleton was also linked to distinct components of the intercellular junctions, It is notable that tropomyosin and a-actinin, which in muscle cells are regulatory proteins of contractile activity, and myosin II are associated with the pericanalicular actin microfilament band; it is the BC counterpart of the contractile actin filament band found in the apical region of other secretory cells, The outer sheath of noncontractile intermediate filaments likely stabilizes the canalicular compartment.