Fine mapping of Ren3 reveals two loci mediating hypersensitive response against Erysiphe necator in grapevine
Fine mapping of Ren3 reveals two loci mediating hypersensitive response against Erysiphe necator in grapevine
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DOI：
10.1007/s10681-017-1857-9
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发表时间：
2017-03-01
期刊：
影响因子：
1.9
通讯作者：
Zyprian, Eva
中科院分区：
文献类型：
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作者：
Zendler, Daniel;Schneider, Pierre;Zyprian, Eva
葡萄藤（Vitis vinifera L.）在经济上对葡萄酒、鲜食葡萄和葡萄干的生产非常重要。然而，葡萄藤受到一系列病原体的威胁。由子囊菌引起的白粉病是世界范围内的一种破坏性疾病。在葡萄品种“Regent”中，先前报道了一个对necator e的抗性位点Ren3。它在15号染色体上的间隔约为7mb。我们试图划定这个区间，以便于进一步的分子分析。设计了新的针对Ren3区的简单序列重复标记。将它们应用于‘Regent’9‘Lemberger’和‘Regent’9‘Cabernet Sauvignon’的杂交群体中，进行了对赤霞珠赤霞珠的抗性分离。此外，我们在2015年和2016年植被期的不同时间点对‘Regent’ 9 ‘Lemberger’后代的E. necator感染水平进行了评分。随后的QTL分析显示，最大LOD值在整个季节从标记GF15-10（位于2.2 Mb）转移到标记GF15-53（位于3.5 Mb）和标记ScORA7*（位于9.4 Mb）（根据PN40024葡萄参考基因组的位置）。为了研究ren3编码的抗性机制，我们进行了离体叶片侵染的显微研究。这些结果表明，携带Ren3的个体会产生超敏反应。对Ren3基因座重组体的离体叶片分析结果表明，在第15号染色体上不仅有一个，而且有两个不同的遗传区域介导了对necator的超敏反应。
Grapevine (Vitis vinifera L.) is economically very important for the production of wine, table grapes and raisins. However, grapevine is threatened by a brought range of pathogens. A destructive disease worldwide is powdery mildew caused by the ascomycete Erysiphe necator. In the grapevine cultivar 'Regent' a resistance locus against E. necator, Ren3, was previously reported. It spans an interval of approximately seven Mb on chromosome 15. We attempted to delimit this interval to facilitate its further molecular analysis. New simple sequence repeat markers targeted to the Ren3 region were designed. They were applied for fine mapping in the cross populations of 'Regent' 9 'Lemberger' and 'Regent' 9 'Cabernet Sauvignon' that segregate for E. necator resistance. Complementarily we scored E. necator infection levels of 'Regent' 9 'Lemberger' progeny at different time points over the course of the vegetation period in 2015 and 2016. Subsequent QTL analysis revealed a maximum LOD value that shifted during the season from marker GF15-10 located at 2.2 Mb to marker GF15-53 located at 3.5 Mb and to marker ScORA7* located at 9.4 Mb on chromosome 15 (positions according to the grapevine reference genome of PN40024). To investigate the Ren3-encoded resistance mechanism we performed detached leaf infection assays for microscopic studies. These revealed that Ren3 carrying individuals react with a hypersensitive response. Results of detached leaf assays on recombinants in the Ren3 locus indicate that not only one, but two distinct genetic regions on chromosome 15 mediate hypersensitive response against E. necator.