Mesoangioblasts, Vessel-Associated Multipotent Stem Cells, Repair the Infarcted Heart by Multiple Cellular Mechanisms
Mesoangioblasts, Vessel-Associated Multipotent Stem Cells, Repair the Infarcted Heart by Multiple Cellular Mechanisms
复制标题
中成血管细胞、血管相关多能干细胞通过多种细胞机制修复梗塞心脏
DOI：
--
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
RobertoLatini
中科院分区：
文献类型：
--
作者：
DanielaGalli;AnnaInnocenzi;LidiaStaszewsky;LuciaZanetta;MaurilioSampaolesi;AntonioBai;ElenaMartinoli;EleonoraCarlo;GiovannaBalconi;FabioFiordaliso;StefanoChimenti;GabriellaCusella;ElisabettaDejana;GiulioCossu;RobertoLatini
目的：与骨髓祖细胞（BMPCs）、成纤维细胞（Fbs）和胚胎干细胞来源的内皮细胞（ECs）相比，测试成血管细胞（Mabs）在减少缺血后损伤方面的潜力，并确定可能的细胞保护机制。方法与结果-冠脉结扎后3 ~ 6小时经皮注射细胞于C57BL/6小鼠左心室，2天和6周后在心脏中进行检测。6周时进行超声心动图检查。单克隆抗体和BMPCs可降低左室扩张，提高左室缩短分数，但ECs和Fbs无此作用。供体细胞在宿主心肌的定植是适度的，主要在血管平滑肌层。单抗组和BMPCs组外周梗死区毛细血管密度较高，心肌细胞凋亡较少。单克隆抗体和BMPCs，而非Fbs或ECs，能促进培养的低氧心肌细胞的存活。这个活动出现在……
Objective— To test the potential of mesoangioblasts (Mabs) in reducing postischemic injury in comparison with bone marrow progenitor cells (BMPCs), fibroblasts (Fbs), and embryonic stem cell–derived endothelial cells (ECs), and to identify putative cellular protective mechanisms. Methods and Results— Cells were injected percutaneously in the left ventricular (LV) chamber of C57BL/6 mice, 3 to 6 hours after coronary ligation, and detected in the hearts 2 days and 6 weeks later. Echocardiographic examinations were performed at 6 weeks. LV dilation was reduced and LV shortening fraction was improved with Mabs and BMPCs but not with ECs and Fbs. Donor cell colonization of the host myocardium was modest and predominantly in the smooth muscle layer of vessels. Capillary density was higher in the peripheral infarct area and apoptotic cardiomyocytes were fewer with Mabs and BMPCs. Mabs and BMPCs, but not Fbs or ECs, promoted survival of cultured cardiocytes under low-oxygen in culture. This activity was present i...