Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria
Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria
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DOI：
10.1007/s00253-005-0073-z
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发表时间：
2006-04-01
影响因子：
5
通讯作者：
Matsushita, K
中科院分区：
文献类型：
--
作者：
Trcek, J;Toyama, H;Matsushita, K
在这项研究中，我们比较了吡咯喹啉醌（PQQ）依赖的乙醇脱氢酶（ADH）的高度耐乙酸菌株的乙酸细菌的生长特性和分子特征。佐治亚州欧洲葡萄对乙酸的抗性最高（10%），而Ga.中间体和巴氏醋酸杆菌抵抗高达6%的乙酸。在不同乙酸浓度的培养基中，Ga.随着乙酸浓度的增加，欧洲对虾的比生长速率逐渐下降。A.与Ga的滞后期相比，pasteurianus的滞后期长两倍和四倍。europaeus和Ga.中间体，分别。PQQ依赖的ADH活性是Ga的两倍。europaeus和Ga. intermedius如A.巴氏杆菌纯化后的酶比活力基本相同，但在乙酸存在下，A. pasteurianus和Ga. intermedius比Ga.欧罗巴。这些结果表明，高ADH活性的Ga。Europaeus细胞和纯化酶的高乙酸稳定性代表了使该物种能够在极高浓度的乙酸下生长并保持代谢活性的两个独特特征。
In this study, we compared the growth properties and molecular characteristics of pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenase (ADH) among highly acetic acid-resistant strains of acetic acid bacteria. Ga. europaeus exhibited the highest resistance to acetic acid (10%), whereas Ga. intermedius and Acetobacter pasteurianus resisted up to 6% of acetic acid. In media with different concentrations of acetic acid, the maximal acetic acid production rate of Ga. europaeus slowly increased, but specific growth rates decreased concomitant with increased concentration of acetic acid in medium. The lag phase of A. pasteurianus was twice and four times longer in comparison to the lag phases of Ga. europaeus and Ga. intermedius, respectively. PQQ-dependent ADH activity was twice as high in Ga. europaeus and Ga. intermedius as in A. pasteurinus. The purified enzymes showed almost the same specific activity to each other, but in the presence of acetic acid, the enzyme activity decreased faster in A. pasteurianus and Ga. intermedius than in Ga. europaeus. These results suggest that high ADH activity in the Ga. europaeus cells and high acetic acid stability of the purified enzyme represent two of the unique features that enable this species to grow and stay metabolically active at extremely high concentrations of acetic acid.