Box-shaped ribozyme octamer formed by face-to-face dimerization of a pair of square-shaped ribozyme tetramers
Box-shaped ribozyme octamer formed by face-to-face dimerization of a pair of square-shaped ribozyme tetramers
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DOI：
10.1016/j.jbiosc.2022.06.008
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发表时间：
2022-09-10
影响因子：
2.8
通讯作者：
Ikawa, Yoshiya
中科院分区：
文献类型：
--
作者：
Islam, Md Dobirul;Hidaka, Kumi;Ikawa, Yoshiya
具有确定的三维（3D）结构的天然存在的核酶作为用于设计和构建人工RNA纳米结构的有前途的平台。我们构建了一个六聚体核酶纳米结构的一对三角形核酶三聚体，其中单位RNA来自四膜虫组I核酶面对面的二聚体。在这项研究中，我们已经扩大了二聚化策略的方形核酶四聚体通过引入四个支柱单位。由此产生的盒形纳米结构，其中包含八个核酶单元，可以从其单元RNA的四个或两个组件组装。(c)2022年，生物技术学会，日本。All rights reserved.
Naturally occurring ribozymes with defined three-dimensional (3D) structures serve as promising platforms for the design and construction of artificial RNA nanostructures. We constructed a hexameric ribozyme nanostructure by face-to-face dimerization of a pair of triangular ribozyme trimers, unit RNAs of which were derived from the Tetrahymena group I ribozyme. In this study, we have expanded the dimerization strategy to a square-shaped ribozyme tetramer by introducing four pillar units. The resulting box-shaped nanostructures, which contained eight ribozyme units, can be assembled from either four or two components of their unit RNAs.(c) 2022, The Society for Biotechnology, Japan. All rights reserved.