SYNAPTIC PLASTICITY IN RAT HIPPOCAMPAL SLICE CULTURES - LOCAL HEBBIAN CONJUNCTION OF PRESYNAPTIC AND POSTSYNAPTIC STIMULATION LEADS TO DISTRIBUTED SYNAPTIC ENHANCEMENT
SYNAPTIC PLASTICITY IN RAT HIPPOCAMPAL SLICE CULTURES - LOCAL HEBBIAN CONJUNCTION OF PRESYNAPTIC AND POSTSYNAPTIC STIMULATION LEADS TO DISTRIBUTED SYNAPTIC ENHANCEMENT
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DOI：
10.1073/pnas.86.20.8113
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发表时间：
1989-10-01
影响因子：
11.1
通讯作者：
AERTSEN, A
中科院分区：
文献类型：
--
作者：
BONHOEFFER, T;STAIGER, V;AERTSEN, A
神经生物学的一个中心主题是寻找学习和记忆的潜在机制。自从卡哈尔和赫布的开创性工作以来，突触被认为是基本的“储存单位”。赫布提出，信息是通过相关性存储的：神经元之间的突触，通常是相互作用的，被增强了。最近的几项发现表明，这种机制确实在中枢神经系统中起作用。突触前纤维的活动与强烈的突触后去极化配对导致突触增强。虽然有大量的证据支持突触后位点的检测同步的前和突触后事件和随后的启动突触增强，这种增强的位点和其随后的持久性仍然是有争议的：前和突触后的贡献已经贝内证明。在所有以前的研究中，增强被假定为特定于突触，其中同步突触前和突触后刺激被应用。我们在这里报告，两种记录技术-光学记录，使用电压敏感染料，和双细胞内记录-揭示，突触增强并不局限于受刺激的细胞。虽然我们配对单传入齐射与细胞内刺激仅限于一个突触后细胞，我们发现，加强也发生在突触之间的刺激突触前纤维和相邻细胞。这表明，突触增强的“配对刺激范例”是不是本地的突触前轴突，事实上，许多相邻的突触后细胞的突触增强。
A central theme in neurobiology is the search for the mechanisms underlying learning and memory. Since the seminal work, first of Cajal and later of Hebb, the synapse is thought to be the basic "storing unit." Hebb proposed that information is stored by correlation: synapses between neurons, which are often coactive, are enhanced. Several recent findings suggest that such a mechanism is indeed operative in the central nervous system. Pairing of activity on presynaptic fibers with strong postsynaptic depolarization results in synaptic enhancement. While there is substantial evidence in favor of a postsynaptic locus for detection of the synchronous pre-and postsyanptic event and subsequent initiation of synaptic enhancement, the locus of this enhancement and its ensuing persistence is still disputed: both pre- and postsynaptic contributions have bene suggested. In all previous studies, the enhancement was presumed to be specific to the synapses where synchronous pre- and postsynaptic stimulation was applied. We report here that two recording techniques-optical recording, using voltage-sensitive dyes, and double intracellular recordings-reveal that synaptic enhacement is not restricted to the stimulated cell. Although we paired single afferent volleys with intracellular stimulation confined to one postsynaptic cell, we found that strengthening also occurred on synapses between the stimulated presynaptic fibers and neighboring cells. This suggests that synaptic enhancement by the "paired-stimulation paradigm" is not local on the presynaptic axons and that, in fact, the synapses of many neighboring postsynaptic cells are enhanced.