Effect of antioxidants and associate changes in antioxidant enzymes in controlling browning and necrosis of proliferating shoots of elite Jatropha curcas L
Effect of antioxidants and associate changes in antioxidant enzymes in controlling browning and necrosis of proliferating shoots of elite Jatropha curcas L
复制标题
DOI：
10.1016/j.biombioe.2010.07.027
复制
发表时间：
2010-12
影响因子：
6
通讯作者：
P. Misra;D. D. Toppo-D.;N. Gupta;D. Chakrabarty;R. Tuli
中科院分区：
文献类型：
--
作者：
P. Misra;D. D. Toppo-D.;N. Gupta;D. Chakrabarty;R. Tuli
在无菌条件下，以田间生长的小桐子节段为外植体，建立了高产优良植株。芽在MS培养基中增殖，该培养基补充有0.5 mg dm− 3苄基腺嘌呤和0.1 mg dm− 3吲哚丁酸沿着有10 mg dm− 3硫酸腺嘌呤以及各15 mg dm− 3的L-谷氨酰胺和L-精氨酸的组合。然而，在培养孵育的15-20天内，观察到组织布朗宁/坏死，导致体外植物再生不良。一组不同的抗氧化剂，即还原型谷胱甘肽，抗坏血酸，生育酚和半胱氨酸单独和组合用于培养基中，以解决组织布朗宁和坏死的问题。抗氧化剂的添加证明有利于新梢的生长。最佳培养基由25 mg dm-3还原型谷胱甘肽和10 mg dm-3抗坏血酸组成，其中增殖的芽具有最高的叶冠面积，保持新鲜，绿色和再生长达40 d的培养孵育，无需任何继代。抗氧化酶的活性，如超氧化物歧化酶在控制芽较高，表明组织布朗宁/坏死与氧化胁迫，这是进一步支持了较高的含量的H2 O2和酚类物质在控制芽相比，其他处理。类似地，谷胱甘肽还原酶、抗坏血酸过氧化物酶和愈创木酚过氧化物酶在处理的芽中高于对照，表明这些芽已经形成了抗氧化酶保护系统，其决定了在氧化胁迫中存活的能力，并且这些酶的上调将有助于减少活性氧的积累。
A high yielding elite plant of Jatropha curcas was established under aseptic condition from field-grown nodal explants. Shoots were proliferated in MS medium supplemented with 0.5 mg dm−3benzyladenine and 0.1 mg dm−3indolebutyric acid along with 10 mg dm−3adenine sulphate and a combination of 15 mg dm−3each of l-glutamine and l-arginine. However, within 15–20 d of culture incubation, tissue browning/necrosis leading to poor plant regeneration in vitro was observed. A set of different antioxidants, namely, reduced glutathione, ascorbic acid, tocopherol and cysteine were used in the medium individually and in combination to solve the problem of tissue browning and necrosis. The addition of antioxidants proved beneficial for the growth of the shoots. The optimum medium comprised of 25 mg dm−3reduced glutathione and 10 mg dm−3ascorbic acid, where proliferating shoots having highest leaf canopy area, remained fresh, green and regenerative up to 40 d of culture incubation without any subculture. The activities of antioxidant enzymes, such as superoxide dismutase was higher in control shoots, indicating that tissue browning/necrosis was associated with oxidative stress which was further supported by higher contents of H2O2and phenolics in control shoots compared to the other treatments. Similarly glutathione reductase, ascorbate peroxidase and guiacol peroxidase was higher in treated shoots than control indicating that these shoots have developed antioxidant enzymatic protective system which determine the ability to survive in oxidative stress and up regulation of these enzymes would help to reduce the built up of reactive oxygen species.