Water and Trehalose: Flow Much Do They Interact with Each Other?
Water and Trehalose: Flow Much Do They Interact with Each Other?
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DOI：
10.1021/jp911940h
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发表时间：
2010-04-15
影响因子：
3.3
通讯作者：
Ricci, M. A.
中科院分区：
文献类型：
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作者：
Pagnotta, S. E.;McLain, S. E.;Ricci, M. A.
早期博物学家的观察发现，一些有机体能够忍受极端的环境条件，并且“享受在死亡后真正复活的优势”[Spallanzani， M. Opuscules de vertebrate, animet Vegetate, 1776]（由Senebier， J. Opuscules翻译自意大利语）。[生物学报，1787,2,203-285]激发了至今仍在继续的研究。隐生，指生物体对不利环境，如脱水和低温的耐受能力。仍然代表着一个未解决的令人着迷的问题。研究表明，许多糖作为生物保护剂发挥着重要的作用，其中表现最好的是双糖海藻糖。目前的假设将海藻糖的保护作用与糖水合壳中水分子的四面体排列的强烈修饰联系起来，海藻糖与溶剂形成了许多氢键。在这里，我们表明，通过最先进的中子衍射实验结合EPSR模拟，海藻糖溶剂化诱导非常微小的水结构的改变。此外，与糖成氢键的水分子数量少得惊人。
The observation made by early naturalists that some organisms could tolerate extreme environmental condisions and "enjoy the advantage of real resurrection alter death" [Spallanzani, M. Opuscules de Physique Animate et Vegetate 1776 (translated from Italian by Senebier, J. Opuscules. de Physique Animate et Vegetate 1787, 2, 203-285)] stimulated research that still continues to this day. Cryptobiosis, the ability of an organism to tolerate adverse environments, such as dehydration and low temperatures. still represents an unsolved and fascinating problem. It has been shown that many sugars play an important role as bioprotectant agents, and among the best performers is the disaccharide trehalose. The current hypothesis links the efficiency of its protective role to strong modifications of the tetrahedral arrangement of water molecules in the sugar hydration shell, with trehalose Conning many hydrogen bonds with the solvent. Here, we show, by means of state-of-the-art neutron diffraction experiments combined with EPSR simulations, that trehalose solvation induces very minor modifications of the water structure. Moreover, the number of water molecules hydrogen-bonded to the sugar is surprisingly small.