Spectrum of light quasi-elastically scattered from suspensions of tobacco mosaic virus. Experimental study of anisotropy in translational diffusion
Spectrum of light quasi-elastically scattered from suspensions of tobacco mosaic virus. Experimental study of anisotropy in translational diffusion
复制标题
烟草花叶病毒悬浮液准弹性散射的光谱。
DOI：
10.1021/ma00140a038
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发表时间：
1984
期刊：
影响因子：
5.5
通讯作者：
Y. Tominaga
中科院分区：
文献类型：
--
作者：
K. Kubota;H. Urabe;Y. Tominaga
本文报道了烟草花叶病毒在10~（-3）M Na_2HPO_4缓冲溶液（pH7.5）中的准弹性光散射实验。理论公式（Maeda，T.; Fujime，S. Macromolecules 1984，17，1157）的一个薄的刚性杆，包括各向异性的平移扩散进行了彻底检查的累积量和双指数分析的相关函数。累积量分析证实了最近的结果（Wilcoxon，J.; Schurr，J. M. Biopolymers 1983，22，849）。双指数分析的结果表明，与理论吻合较好。分析是高度一致的，彼此和阐明了平移扩散的动态光散射光谱的各向异性的大的影响。研究了关联函数随动量传递矢量大小的变化规律，实验关联函数与理论关联函数符合较好。本文的结果表明，旋转扩散系数和横向和纵向平移扩散系数可以同时由动态光散射测量确定。
Quasi-elastic light scattering experiments are reported on tobacco mosaic virusin 10~ 3 M Na2HP04 buffer solution (pH 7.5). The theoretical formulation (Maeda, T.; Fujime, S. Macromolecules 1984, 17, 1157) for a thin rigid rod including anisotropy in translational diffusion was thoroughly examined by cumulant and two-exponential analyses of correlation functions. The cumulant analysis confirmed a recent result (Wilcoxon, J.; Schurr, J. M. Biopolymers 1983, 22,849). The results of the two-exponential analysis showed a good agreement with the theory. The analyses were highly consistent with each other and elucidated a large effect of anisotropy in translational diffusion on dynamic light scattering spectra. The behaviors of correlation functions against the magnitude of the momentumtransfer vector were examined, and a good agreement was obtained between experimental and theoretical correlation functions. The present results implythat boththe rotational diffusion coefficient and the sideways and lengthways translational diffusion coefficients can be determined simultaneously from dynamic light scatteringmeasurements.