Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase.
Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase.
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DOI：
10.1016/j.jbiotec.2007.04.019
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发表时间：
2007-06
影响因子：
4.1
通讯作者：
Seiya Watanabe;A. Saleh;S. Pack;N. Annaluru;T. Kodaki;K. Makino
中科院分区：
文献类型：
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作者：
Seiya Watanabe;A. Saleh;S. Pack;N. Annaluru;T. Kodaki;K. Makino
使用与来自树干毕赤酵母的天然木糖还原酶一起表达的重组酿酒酵母来评估来自树干毕赤酵母的木糖醇脱氢酶（XDH）对NADP+的逆转辅酶特异性和热稳定性对木糖发酵成乙醇的影响。突变后的XDH具有与S.与体外培养的酿酒酵母细胞相比，在表达NADP+依赖性XDH的Y-ARSdR菌株中发现显著增强;与表达野生型XDH的参考菌株相比，不利的木糖醇分泌减少86%，乙醇产量增加41%。
Effects of reversal coenzyme specificity toward NADP+and thermostabilization of xylitol dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were estimated using a recombinant Saccharomyces cerevisiae expressing together with a native xylose reductase from P. stipitis. The mutated XDHs performed the similar enzyme properties in S. cerevisiae cells, compared with those in vitro. The significant enhancement(s) was found in Y-ARSdR strain, in which NADP+-dependent XDH was expressed; 86% decrease of unfavorable xylitol excretion with 41% increased ethanol production, when compared with the reference strain expressing the wild-type XDH.