The E. coli L-asparaginase V27T mutant: structural and functional characterization and comparison with theoretical predictions.
The E. coli L-asparaginase V27T mutant: structural and functional characterization and comparison with theoretical predictions.
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DOI：
10.1002/1873-3468.14526
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发表时间：
2022-12
期刊：
影响因子：
3.5
通讯作者：
Lubkowski J
中科院分区：
文献类型：
--
作者：
Strzelczyk P;Zhang D;Wlodawer A;Lubkowski J
Bacterial L-asparaginases have been used for over 40 years as anticancer drugs. Ardalan et al. (Medical Hypotheses 112, 7–17, 2018) proposed that the V27T mutant of Escherichia coli type II L-asparaginase, EcAII(V27T), should display altered biophysical and catalytic properties compared to the wild-type enzyme, EcAII(wt), rendering it more favourable as a pharmaceutical. They postulated that EcAII(V27T) would exhibit reduced glutaminolytic activity and be more stable compared to EcAII(wt). Their postulates, however, were purely theoretical. Here, we characterized experimentally selected properties of EcAII(V27T). We found asparaginolytic activity of this mutant unchanged, whereas its glutaminolytic activity was fourfold lower compared with EcAII(wt). We did not observe significant differences in stabilities of EcAII(wt) and EcAII(V27T). Crystal structures of the complexes with L-Asp and L-Glu showed considerable differences in binding modes of both substrates.
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DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1007/978-981-13-7709-9_9
发表时间：
2019-01-01
期刊：
THERAPEUTIC ENZYMES: FUNCTION AND CLINICAL IMPLICATIONS
影响因子：
--
作者：
Maggi, Maristella;Scotti, Claudia
通讯作者：
Scotti, Claudia
影响因子：
3.9
作者：
ROHM, KH;VANETTEN, RL
通讯作者：
VANETTEN, RL
影响因子：
0.2
作者：
Egler RA;Ahuja SP;Matloub Y
通讯作者：
Matloub Y
影响因子：
4.6
作者：
Strzelczyk P;Zhang D;Dyba M;Wlodawer A;Lubkowski J
通讯作者：
Lubkowski J