Endothelial Colony-Forming Cells in Young Adults Born Preterm: A Novel Link Between Neonatal Complications and Adult Risks for Cardiovascular Disease.
Endothelial Colony-Forming Cells in Young Adults Born Preterm: A Novel Link Between Neonatal Complications and Adult Risks for Cardiovascular Disease.
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DOI：
10.1161/jaha.118.009720
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发表时间：
2018-07-09
影响因子：
5.4
通讯作者：
Nuyt AM
中科院分区：
文献类型：
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作者：
Bertagnolli M;Xie LF;Paquette K;He Y;Cloutier A;Fernandes RO;Béland C;Sutherland MR;Delfrate J;Curnier D;Bigras JL;Rivard A;Thébaud B;Luu TM;Nuyt AM
早产与心血管风险和疾病有关。内皮祖细胞在血管发育和修复中发挥着关键作用。早产儿的脐带血内皮祖细胞，尤其是内皮集落形成细胞（ECFC），对早产相关的促氧化应激的敏感性增强。早产后成年期是否存在 ECFC 功能障碍尚不清楚。这项横断面观察性研究包括 55 名早产（≤ 29 孕周）的年轻人（18-29 岁，38% 男性）和 55 名性别和年龄匹配的足月对照。从外周血中分离出ECFC；在体外评估细胞增殖和血管形成能力。早产儿的白天收缩压较高，而葡萄糖耐量和体重指数较低。 62% 的足月参与者和 58% 的早产参与者的 ECFC 菌落在培养物中生长。早产参与者在培养后期形成了 ECFC 集落，并且与对照组相比增殖减少。仅在早产个体中，我们观察到 ECFC 集落在培养物中生长得越晚，整体 ECFC 功能就越差。此外，在早产儿中，收缩压升高与 ECFC 增殖减少（rS=-0.463；P=0.030）和基质胶上形成的分支数量显着相关（rS=-0.443；P=0.039）。在早产受试者中，支气管肺发育不良与 ECFC 功能受损相关，而接触产前类固醇则与 ECFC 功能改善相关。这项研究是第一个检查早产成人 ECFC 的研究，并证明与足月对照相比 ECFC 功能障碍。在早产组中，ECFC 功能障碍与支气管肺发育不良（主要的早产儿相关新生儿发病率）以及成年期收缩压升高有关。
Preterm birth is linked to cardiovascular risks and diseases. Endothelial progenitor cells play a critical role in vascular development and repair. Cord blood endothelial progenitor cells of preterm‐born infants, especially endothelial colony‐forming cells (ECFC), show enhanced susceptibility to prematurity‐related pro‐oxidant stress. Whether ECFC dysfunction is present in adulthood following preterm birth is unknown. This cross‐sectional observational study includes 55 preterm‐born (≤29 gestational weeks) young adults (18–29 years old, 38% male) and 55 sex‐ and age‐matched full‐term controls. ECFC were isolated from peripheral blood; cell proliferative and vascular cord formation capacities were assessed in vitro. Daytime systolic blood pressure was higher, whereas glucose tolerance and body mass index were lower in preterm‐born subjects. ECFC colonies grew in culture for 62% of full‐term‐ and 58% of preterm‐born participants. Preterm‐born participants have formed ECFC colonies later in culture and have reduced proliferation compared with controls. Only in preterm‐born individuals, we observed that the later the ECFC colony grows in culture, the worse was overall ECFC function. In addition, in preterms, elevated systolic blood pressure significantly correlated with reduced ECFC proliferation (rS=−0.463; P=0.030) and numbers of branches formed on matrigel (rS=−0.443; P=0.039). In preterm‐born subjects, bronchopulmonary dysplasia was associated with impaired ECFC function, whereas exposure to antenatal steroids related to better ECFC function. This study is the first to examine ECFC in preterm‐born adults and to demonstrate ECFC dysfunction compared with full‐term controls. In the preterm‐born group, ECFC dysfunction was associated with bronchopulmonary dysplasia, the major prematurity‐related neonatal morbidity, and with increased systolic blood pressure into adulthood.