Enhanced bone regeneration by electrical polarization of hydroxyapatite
Enhanced bone regeneration by electrical polarization of hydroxyapatite
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DOI：
10.1111/j.1525-1594.2006.00313.x
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发表时间：
2006-11-01
影响因子：
2.4
通讯作者：
Yamashita, Kimihiro
中科院分区：
文献类型：
--
作者：
Itoh, Soichiro;Nakamura, Satoshi;Yamashita, Kimihiro
我们研究了成骨细胞的反应和新骨形成的电极化羟基磷灰石（HAp）板植入到大鼠颅骨骨缺损。在第一组大鼠中，将测试件以其带正电荷的表面面朝下放置在硬脑膜上。在第二组中，将测试件以其带负电荷的表面面朝下放置在硬脑膜上。第三组接受不带电的测试片。进行组织学检查以表征新形成的骨以及定量。酶组织化学检测碱性磷酸酶和抗酒石酸酸性磷酸酶，定量成骨细胞活性。在接受极化HAp板的组中，在带负电荷和正电荷的表面上，骨生长得到增强。除了静电力将Ca 2+吸引到带负电荷的表面之外，对带正电荷的表面上的破骨细胞增殖的抑制作用可以增强骨形成。
We examined osteogenic cell responses and new bone formation on electrically polarized hydroxyapatite (HAp) plates implanted into calvarial bone defects in rats. In the first group of rats, test pieces were placed with their positively charged surfaces face down on the dura mater. In a second group, test pieces were placed with their negatively charged surfaces facedown on the dura mater. A third group received noncharged test pieces. Histological examination was carried out to characterize the newly formed bone as well as quantification. Enzyme histochemistry involving the detection of alkaline phosphatase and tartrate-resistant acid phosphatase was performed to quantify osteogenic cell activity. Bone growth was enhanced in the groups that received polarized HAp plates, both at the negatively and positively charged surfaces. In addition to the electrostatic force that attracts Ca2+ to the negatively charged surfaces, the suppressive effects on osteoclasts proliferation on the positively charged surfaces may enhance bone formation.