Detection of bladder cancer recurrence by microsatellite analysis of urine
Detection of bladder cancer recurrence by microsatellite analysis of urine
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DOI：
10.1038/nm0697-621
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发表时间：
1997-06-01
期刊：
影响因子：
82.9
通讯作者：
Sidransky, D
中科院分区：
文献类型：
--
作者：
Steiner, G;Schoenberg, MP;Sidransky, D
一种可靠的、无创的监测膀胱移行细胞癌（TCC）的方法将有很大的临床价值。膀胱镜检查是目前的“黄金标准”，但它是侵入性的，昂贵的，而且对病人来说不舒服。最近，我们展示了一种基于尿液DNA微卫星分析的原发性膀胱癌检测新方法。为了确定该技术在TCC患者随访中的可行性，我们采用盲法检测了21名接受膀胱癌治疗的患者的尿液样本，这些患者含有20种多态微卫星标记物。我们在11例患者中检测到10例复发病变，并在膀胱镜检查肿瘤证据的4个月和6个月前正确预测了2例患者尿液中肿瘤细胞群的存在。10例无明显癌症的患者中有10例检测结果为阴性。尿液沉积物的微卫星分析是一种检测复发性膀胱癌的新型且潜在的强大临床工具。
A reliable, noninvasive method for monitoring patients with transitional cell carcinoma (TCC) of the bladder would be of great clinical benefit. Cystoscopy is currently the ''gold standard,'' but it is invasive, expensive and uncomfortable for the patient. Recently, we demonstrated a novel approach for the detection of primary bladder cancer based on microsatellite analysis of urine DNA. To determine the feasibility of this technique for following-up patients with TCC, we tested serial urine samples from 21 patients who had been treated for bladder cancer with 20 polymorphic microsatellite markers in a blinded fashion. We detected recurrent lesions in 10 out of 11 patients and correctly predicted the existence of a neoplastic cell population in the urine of two patients, 4 and 6 months before cystoscopic evidence of the tumor. The assay was negative in 10 of 10 patients who had no evident cancer. Microsatellite analysis of urine sediment represents a novel and potentially powerful clinical tool for the detection of recurrent bladder cancer.